Impaired Vibration of Auditory Ossicles in Osteopetrotic Mice
Impaired Vibration of Auditory Ossicles in Osteopetrotic Mice
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DOI：
10.1016/j.ajpath.2010.11.063
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发表时间：
2011-03-01
影响因子：
6
通讯作者：
Matsuo, Koichi
中科院分区：
文献类型：
--
作者：
Kanzaki, Sho;Takada, Yasunari;Matsuo, Koichi
在中耳，由三块小骨头组成的链条（即锤骨、砧骨和镫骨）通过振动将声音从鼓膜传递到内耳。关于骨吸收破骨细胞是否以及如何在听小骨的振动中起作用，我们知之甚少。我们分析了由于细胞因子RANKL或转录因子c-Fos缺乏破骨细胞而导致的骨质疏松小鼠的听力功能和听骨形态学特征。听觉脑干反应表明，两种基因型的小鼠都经历了听力损失，激光多普勒振动仪显示，鼓膜后的鼓槌没有振动。组织学分析和同步辐射x线断层扫描显示，骨质疏松小鼠的听小骨比对照小鼠的听小骨更厚，软骨更多。最有趣的是，在骨质疏松小鼠中，短柄柄接触鼓室内壁，c-Src激酶缺陷小鼠（非吸收破骨细胞数量正常）也是如此。与对照组相比，骨质疏松小鼠的鼓室体积较小，但听骨较大。这些数据表明破骨细胞骨吸收是听骨变薄和鼓室增大所必需的，从而使听骨自由振动。[J] .中华病理学杂志，2011,38(2):1270-1274。DOI: 10.1016 / j.ajpath.2010.11.063)
In the middle ear, a chain of three tiny bones (ie, malleus, incus, and stapes) vibrates to transmit sound from the tympanic membrane to the inner ear. Little is known about whether and how bone-resorbing osteoclasts play a role in the vibration of auditory ossicles. We analyzed hearing function and morphological features of auditory ossicles in osteopetrotic mice, which lack osteoclasts because of the deficiency of either cytokine RANKL or transcription factor c-Fos. The auditory brainstem response showed that mice of both genotypes experienced hearing loss, and laser Doppler vibrometry revealed that the malleus behind the tympanic membrane failed to vibrate. Histological analysis and X-ray tomographic microscopy using synchrotron radiation showed that auditory ossicles in osteopetrotic mice were thicker and more cartilaginous than those in control mice. Most interestingly, the malleal processus brevis touched the medial wall of the tympanic cavity in osteopetrotic mice, which was also the case for c-Src kinase deficient mice (with normal numbers of nonresorbing osteoclasts). Osteopetrotic mice showed a smaller volume of the tympanic cavity but had larger auditory ossicles compared with controls. These data suggest that osteoclastic bone resorption is required for thinning of auditory ossicles and enlargement of the tympanic cavity so that auditory ossicles vibrate freely. (Am J Pathol 2011, 178:1270-1274. DOI: 10.1016/j.ajpath.2010.11.063)