Expression of a cloned rat brain potassium channel in Xenopus oocytes.
Expression of a cloned rat brain potassium channel in Xenopus oocytes.
复制标题
克隆的大鼠脑钾通道在爪蟾卵母细胞中的表达。
DOI：
10.1126/science.2539643
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
North,RA
中科院分区：
文献类型：
--
作者：
Christie,MJ;Adelman,JP;Douglass,J;North,RA
钾通道是神经组织中普遍存在的膜蛋白，在神经组织中具有重要作用，但其功能与分子结构之间的关系却知之甚少。以大鼠海马区为材料，构建互补DNA文库，获得互补DNA克隆(RBK-1)。预测的495个氨基酸的蛋白质序列与果蝇Shakerocus编码的钾通道蛋白同源，与小鼠克隆MBK-1的预期产物只有三个氨基酸的差异。体外转录的rBK-1信使RNA被注射到Xenopus卵母细胞中，指导钾通道的表达。通过表达通道的钾电流类似于哺乳动物神经元中报道的瞬时(或A)和延迟整流电流，并且对4-氨基吡啶和四乙基铵都敏感。
Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by theShakerlocus ofDrosophilaand differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected intoXenopusoocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.