Local FK506 delivery at the direct nerve repair site improves nerve regeneration
Local FK506 delivery at the direct nerve repair site improves nerve regeneration
复制标题
DOI：
10.1002/mus.26656
复制
发表时间：
2019-08-23
期刊：
影响因子：
3.4
通讯作者：
Agarwal, Jay
中科院分区：
文献类型：
--
作者：
Davis, Brett;Hilgart, David;Agarwal, Jay
引言本研究的目的是评估局部他克莫司（FK 506）给药改善神经横断损伤的疗效。方法制备FK 506包埋聚丙交酯-己内酯缓释膜。进行了FK 506释放度检测和生物活性测定。使用小鼠坐骨神经切断和直接修复模型来评估FK 506的延长的局部递送对神经再生结果的影响。结果FK 506的线性释放持续30天，释放的FK 506与背根神经节试验中的神经突起延伸的对照水平相匹配。与非FK 506组相比，局部FK 506治疗组的腓肠肌重量、足肌电图和修复部位远端的轴突数量更大。讨论本研究的结果表明，在小鼠坐骨神经横断和修复中，FK 506对神经损伤的延长局部递送可改善神经再生结果。
Introduction The objective of this study is to assess the efficacy of local tacrolimus (FK506) delivery to improve outcomes in the setting of nerve transection injury. Methods FK506 embedded poly(lactide-co-caprolactone) films capable of extended, localized release of FK506 were developed. FK506 rate of release testing and bioactivity assay was performed. Mouse sciatic nerve transection and direct repair model was used to evaluate the effect extended, local delivery of FK506 had on nerve regeneration outcomes. Results Linear release of FK506 was observed for 30 days and released FK506 matched control levels of neurite extension in the dorsal root ganglion assay. Groups treated with local FK506 had greater gastrocnemius muscle weight, foot electromyogram, and number of axons distal of the repair site than non-FK506 groups. Discussion Results of this study indicate that extended, localized delivery of FK506 to nerve injuries can improve nerve regeneration outcomes in a mouse sciatic nerve transection and repair.