Inertial Manipulation and Transfer of Microparticles Across Laminar Fluid Streams
Inertial Manipulation and Transfer of Microparticles Across Laminar Fluid Streams
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DOI：
10.1002/smll.201200588
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发表时间：
2012-09-10
期刊：
影响因子：
13.3
通讯作者：
Di Carlo, Dino
中科院分区：
文献类型：
--
作者：
Gossett, Daniel R.;Tse, Henry Tat Kwong;Di Carlo, Dino
控制粒子在水流中运动的一般策略将使单细胞分析、固相反应控制和生物物理学研究具有新的能力。通过流转移细胞难以以良好控制的方式实现，因为它需要利用外部力场或精确制造的机械结构来沿着精确控制流体流动。本文介绍了一种基于被动惯性升力的颗粒转移策略，并对具有变化纵横比的通道的这些力的分布进行了改变。独特的是，使用的主导壁效应升力平行于粒子旋转方向的探索和利用，以实现可控的横流运动。以这种方式，颗粒被定位成以超过每秒1000个颗粒的速率和亚毫秒的转移时间迁移穿过层流并进入新的溶液，而不会显著干扰界面。证明了快速惯性溶液交换（RInSE）用于制备血液样品和其他细胞测定的能力。最后，对过量荧光染料的RInSE和下游分析聚焦后的在线流式细胞术的改进进行了表征。所描述的方法简单地应用于操纵细胞和颗粒，并将它们快速暴露于反应溶液或从反应溶液中除去它们，在控制和分析细胞或颗粒上的低亲和力相互作用中具有更广泛的应用。
A general strategy for controlling particle movement across streams would enable new capabilities in single-cell analysis, solid-phase reaction control, and biophysics research. Transferring cells across streams is difficult to achieve in a well-controlled manner, since it requires precise control of fluid flow along with external force fields or precisely manufactured mechanical structures. Herein a strategy is introduced for particle transfer based on passive inertial lift forces and shifts in the distribution of these forces for channels with shifting aspect ratios. Uniquely, use of the dominant wall-effect lift parallel to the particle rotation direction is explored and utilized to achieve controllable cross-stream motion. In this way, particles are positioned to migrate across laminar streams and enter a new solution without significant disturbance of the interface at rates exceeding 1000 particles per second and sub-millisecond transfer times. The capabilities of rapid inertial solution exchange (RInSE) for preparation of hematological samples and other cellular assays are demonstrated. Lastly, improvements to inline flow cytometry after RInSE of excess fluorescent dye and focusing for downstream analysis are characterized. The described approach is simply applied to manipulating cells and particles and quickly exposing them to or removing them from a reacting solution, with broader applications in control and analysis of low affinity interactions on cells or particles.