MONOCLONAL-ANTIBODY ANALYSIS OF MONONUCLEAR-CELLS IN MYOPATHIES .1. QUANTITATION OF SUBSETS ACCORDING TO DIAGNOSIS AND SITES OF ACCUMULATION AND DEMONSTRATION AND COUNTS OF MUSCLE-FIBERS INVADED BY T-CELLS
MONOCLONAL-ANTIBODY ANALYSIS OF MONONUCLEAR-CELLS IN MYOPATHIES .1. QUANTITATION OF SUBSETS ACCORDING TO DIAGNOSIS AND SITES OF ACCUMULATION AND DEMONSTRATION AND COUNTS OF MUSCLE-FIBERS INVADED BY T-CELLS
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DOI：
10.1002/ana.410160206
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发表时间：
1984-01-01
影响因子：
11.2
通讯作者：
ENGEL, AG
中科院分区：
文献类型：
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作者：
ARAHATA, K;ENGEL, AG
在76例肌肉标本（正常对照9例，杜氏营养不良11例，硬皮病11例，皮肌炎13例，多发性肌炎15例，包体性肌炎17例）中，在血管周围、膜周和肌内膜积聚部位分析了单个核细胞。细胞分型采用B细胞、T细胞、T细胞亚群、杀伤细胞(K)或自然杀伤细胞（NK）和Ia抗原的单克隆抗体。通过酸性磷酸酶反应鉴定巨噬细胞。正常肌肉中很少有血管外单个核细胞。在所有炎症性肌病中，T细胞、B细胞和巨噬细胞混合渗出。成熟的K/NK细胞在所有疾病中都很少见。皮肌炎、多发性肌炎和包体体肌炎的T细胞、T8+细胞和活化T细胞呈正梯度，B细胞和T4+细胞呈负梯度。硬皮病中主要的膜周渗出液主要由T细胞和巨噬细胞组成。皮肌炎各部位B细胞百分比及肌内膜T4+/T细胞比值明显高于其他疾病。在多发性肌炎和包涵体肌炎中，肌内膜渗出液中含有大量的T细胞、T8+细胞和活化的T细胞，但只有稀疏的B细胞。在多发性肌炎和包涵体肌炎中，T细胞伴巨噬细胞局部包围和侵袭非坏死纤维。杜氏营养不良症的罕见纤维和皮肌炎和硬皮病的极少数纤维也受到类似的影响。因此，在不同的肌病中，肌肉中可能存在T-B、T-T和t -巨噬细胞的协同作用。T细胞介导的纤维损伤在多发性肌炎和包涵体肌炎中起重要作用。T细胞介导的纤维损伤也可发生在遗传性疾病中，如杜氏营养不良症。皮肌炎患者的肌肉可能发生局部体液反应，多发性肌炎和包涵体肌炎也可能发生局部体液反应。
In 76 muscle specimens (normal controls, 9; Duchenne dystrophy, 11; scleroderma, 11; dermatomyositis, 13; polymyositis, 15; inclusion body myositis, 17), mononuclear cells were analyzed at perivascular, perimysial and endomysial sites of accumulation. Monoclonal antibodies reactive for B cells, T cells T cell subsets, killer (K) or natural killer (NK) cells and the Ia antigen were used for cell typing. Macrophages were identified by the acid phosphatase reaction. Few extravascular mononuclear cells occurred in normal muscle. In all inflammatory myopathies, a mixed exudate of T cells, B cells and macrophages was present. Mature K/NK cells were rare in all diseases. In dermatomyositis, polymyositis and inclusion body myositis, there was a positive gradient for T cells, T8+ cells and activated T cells and a negative gradient for B cells and T4+ cells between perivascular and endomysial sites. In scleroderma the predominant perimysial exudate consisted mostly of T cells and macrophages. The percentage of B cells at all sites, and the T4+/T cell ratio in the endomysium were significantly higher in dermatomyositis than in the other diseases. In polymyositis and inclusion body myositis, the endomysial exudate contained a large number of T cells, T8+ cells and activated T cells but only sparse B cells. T cells accompanied by macrophages focally surrounded and invaded nonnecrotic fibers in polymyositis and inclusion body myositis. Rare fibers in Duchenne dystrophy and a very few fibers in dermatomyositis and scleroderma were similarly affected. Thus, T-B, T-T and T-macrophage cooperativities are likely to exist in muscle in different myopathies. T cell-mediated fiber injury plays a role in polymyositis and inclusion body myositis. T cell-mediated fiber injury can also occur in inherited diseases, such as Duchenne dystrophy. Local humoral response may occur in muscle in dermatomyositis and possibly in polymyositis and inclusion body myositis.