PROHEAD AND DNA-GP3-DEPENDENT ATPASE ACTIVITY OF THE DNA PACKAGING PROTEIN GP16 OF BACTERIOPHAGE-PHI-29
PROHEAD AND DNA-GP3-DEPENDENT ATPASE ACTIVITY OF THE DNA PACKAGING PROTEIN GP16 OF BACTERIOPHAGE-PHI-29
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DOI：
10.1016/0022-2836(87)90121-5
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发表时间：
1987-09-20
影响因子：
5.6
通讯作者：
ANDERSON, D
中科院分区：
文献类型：
--
作者：
GUO, PX;PETERSON, C;ANDERSON, D
在完全确定的体外组装系统中，对vphi.29噬菌体DNA包装蛋白gp16(基因产物16)的ATPase活性进行了研究。在包括纯化的核酸、DNA-GP3和gp16的包装反应中，ATP被水解成ADP和PI。大约一个三磷酸腺苷分子被用于包装两个碱基对的.vphi。DNA，或9倍。每个病毒体103个ATP分子。Gp16对ATP的降解既依赖于前药，又依赖于DNA-GP3。Gp16包含“A-型”和“B-型”的ATP结合共识序列(Walker等人，1982)和预测的ATP结合的二级结构。Gp16的A型序列为“碱性-疏水区-G-X2-G-X-G-K-S-X7-疏水”，与Lambda、T7和T4的噬菌体包装蛋白gp19和gp17序列相似。这些蛋白既有ATP结合结构域，又有潜在的镁结合结构域，它们可能都具有ATPase的功能，并可能具有共同的Prohead结合能力。.vphi。与DNA共价结合的蛋白GP3可能在功能上类似于与DNA结合的lambda蛋白gpNu1和vphi.21的GP1。
The ATPase activity of the DNA packaging protein gp16 (gene product 16) of bacteriophage .vphi.29 was studied in the completely defined in-vitro assembly system. ATP was hydrolyzed to ADP and Pi in the packaging reaction that included purified proheads, DNA-gp3 and gp16. Approximately one molecule of ATP was used in the packaging of 2 base-pairs of .vphi. DNA, or 9 .times. 103 ATP molecules per virion. The hydrolysis of ATP by gp16 was both prohead and DNA-gp3 dependent. gp16 contained both the "A-type" and the "B-type" ATP-binding consensus sequences (Walker et al., 1982) and the predicted secondary structure for ATP binding. The A-type sequence of gp16 was "basic-hydrophobic region-G-X2-G-X-G-K-S-X7-hydrophobic", and similar sequences were found in the phage DNA packaging proteins gpA of lambda, gp19 of T7 and gp17 of T4. Having both the ATP-binding and potential magnesium-binding domains, all of these proteins probably function as ATPases and may have common prohead-binding capabilities. The .vphi. protein gp3, covalently bound to the DNA, may be analogous in function to proteins gpNu1 of lambda and gp1 of .vphi.21 that bind the DNA.