Tank bottom area influences the growth, molting, stress response, and antioxidant capacity of juvenile mud crab Scylla paramamosain
Tank bottom area influences the growth, molting, stress response, and antioxidant capacity of juvenile mud crab Scylla paramamosain
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DOI：
10.1016/j.aquaculture.2021.737705
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发表时间：
2021-11
期刊：
影响因子：
4.5
通讯作者：
Kaijia Yu;Ce Shi;Xizhen Liu;Yangfang Ye;Chunlin Wang;C. Mu;Weiwei Song;Zhiming Ren
中科院分区：
文献类型：
--
作者：
Kaijia Yu;Ce Shi;Xizhen Liu;Yangfang Ye;Chunlin Wang;C. Mu;Weiwei Song;Zhiming Ren
通过为期8周的试验，研究了不同池底面积（TBA）对拟穴青蟹幼蟹生长性能、应激反应和抗氧化能力的影响。四个处理，一式三份，具有不同的k值（k= A/CW 2，其中A是水箱的底部面积（mm 2），CW是幼蟹的甲壳宽度（mm）），即，在本实验中使用k值= 4.42（K4）、17.67（K18）、39.76（K40）和70.68（K70）。结果表明，各处理间成活率差异不显著（83.33-96.67%），但以K40组生长性能最好（P< 0.05）。随着TBA浓度的降低，蜕皮激素和蜕皮激素受体（EcR）基因表达水平降低，而蜕皮抑制激素（MIH）基因表达水平升高。结果，在K4和K18中观察到蜕皮间隔延长和增重率降低。K4组皮质醇、乳酸含量显著高于对照组（P< 0.05），糖原含量显著低于对照组（P< 0.05），磷酸果糖激酶（PFK）和丙酮酸激酶（PK）活性显著高于对照组（P< 0.05）。K4和K18组过氧化氢酶（CAT）活性较低，丙二醛（MDA）含量较高。结果表明，TBA显著影响S. paramamosain，和效果可以通过激素，糖代谢和抗氧化剂途径介导。值得注意的是，根据WGR的二次多项式回归分析，最佳TBA估计为29.23-59.19（k值），并且超大的水箱在生长性能方面不能提供额外的益处。因此，在设计河蟹养殖设施时，应考虑TBA。
An 8-week trial was performed to determine the effects of different tank bottom areas (TBA) on growth performance, stress response, and antioxidant capacity of juvenile mud crabScylla paramamosain. Four treatments in triplicates with differentk-value (k= A/CW2, where A is the bottom area (mm2) of the tank, and CW is the carapace width (mm) of the juvenile crab),i.e.,k-value = 4.42 (K4), 17.67 (K18), 39.76 (K40), and 70.68 (K70), were used in this experiment. The results showed no significant difference in survival rate (83.33–96.67%) was found, but the K40 group had the best growth performance among all the treatments (P< 0.05). With the decrease of TBA, the level of ecdysone and ecdysone receptor (EcR) gene expression decreased, while the molt inhibitory hormone (MIH) gene expression increased. As a result, prolonged molting intervals and reduced weight gain rates were observed in K4 and K18. Higher cortisol, lactic acid, and lower glycogen content were also observed in K4 (P< 0.05), accompanied by higher phosphofructokinase (PFK) and pyruvate kinase (PK) enzyme activities (P< 0.05). On the contrary, lower catalase (CAT) and higher malondialdehyde (MDA) were observed in the K4 and K18 groups. The results demonstrated that TBA significantly impacted the growth performance ofS. paramamosain, and the effects could be mediated through hormonal, glycometabolism, and antioxidant pathways. Notably, the optimal TBA was estimated to be 29.23–59.19 (k-value) according to the quadratic polynomial regression analysis of WGR, and the oversized tank could not give extra benefits in terms of growth performance. Therefore, TBA should be deliberated when designing crab culture facilities.