Air-exposure technique for the formation of artificial lipid bilayers in microsystems
Air-exposure technique for the formation of artificial lipid bilayers in microsystems
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DOI：
10.1021/la7007528
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发表时间：
2007-07-17
期刊：
影响因子：
3.9
通讯作者：
Morgan, Hywel
中科院分区：
文献类型：
--
作者：
Sandison, Mairi E.;Zagnoni, Michele;Morgan, Hywel
为了开发一种可靠的方法，芯片上的双层脂质膜（BLM）的形成，这可以用于在生物传感器阵列平台，聚合物微流控装置已被构建，并已在它的悬浮BLM的形成进行了研究。一个简单的，但可重复的BLM形成协议已被开发，其中一个简短的空气暴露期是用来诱导最初厚的脂质溶剂层的快速变薄。该技术快速、可重复，并且适合蛋白质或分析物的简单注射，以及膜两侧的缓冲液交换。按比例放大用于阵列平台的技术也很简单，同时形成三个单独可寻址的BLM被证明。
To develop a reliable method for on-chip bilayer lipid membrane (BLM) formation, which could be employed for use in a biosensor array platform, a polymer microfluidic device has been constructed, and the formation of suspended BLMs within it has been investigated. A simple, yet reproducible BLM formation protocol has been developed, in which a brief air-exposure period is employed to induce the rapid thinning of an initially thick lipid-solvent layer. The technique is rapid, reproducible, and amenable to the simple injection of proteins or analytes, as well as to buffer exchange on both sides of the membrane. Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated.