Sulfite Oxidation Catalyzed by aa 3-Type Cytochrome c Oxidase in Acidithiobacillus ferrooxidans
Sulfite Oxidation Catalyzed by aa 3-Type Cytochrome c Oxidase in Acidithiobacillus ferrooxidans
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氧化亚铁硫杆菌中aa 3型细胞色素c氧化酶催化的亚硫酸盐氧化
DOI：
10.1271/bbb.100446
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
F. Takeuchi
中科院分区：
文献类型：
--
作者：
T. Sugio;Ami Ako;F. Takeuchi
亚硫酸盐是在氧化亚铁硫杆菌硫氧化过程中作为有毒中间体产生的。氧化亚铁菌 D3-2 具有最高的铜生物浸出活性，比其他氧化亚铁菌菌株（包括 ATCC 23270）对亚硫酸盐具有更强的抵抗力。当从菌株 D3-2 中均匀纯化亚硫酸盐氧化酶时，纯化的亚硫酸盐氧化酶吸收光谱的氧化和还原形式与氧化亚铁菌 aa 3 型细胞色素 c 氧化酶的吸收光谱相对应。纯化的亚硫酸盐氧化酶的α-亚基(52.5 kDa)、β-亚基(25 kDa)和γ-亚基(20 kDa)的确认分子量以及亚硫酸盐氧化酶(AAKKG)的γ-亚基的N-末端氨基酸序列与氧化亚铁曲霉ATCC 23270细胞色素c氧化酶的氨基酸序列相对应。铁和硫生长的氧化亚铁菌D3-2的亚硫酸盐氧化酶活性比从氧化亚铁菌菌株ATCC 23270、MON-1和AP19-3纯化的细胞色素c氧化酶活性高得多。从铁和硫生长的菌株 D3-2 中纯化的亚硫酸盐氧化酶的活性被针对纯化的氧化亚铁菌 MON-1 aa 3 型细胞色素 c 氧化酶产生的抗体完全抑制。这是第一份表明 3 型细胞色素 c 氧化酶催化氧化亚铁菌中亚硫酸盐氧化的报告。
Sulfite is produced as a toxic intermediate during Acidithiobacillus ferrooxidans sulfur oxidation. A. ferrooxidans D3-2, which posseses the highest copper bioleaching activity, is more resistant to sulfite than other A. ferrooxidans strains, including ATCC 23270. When sulfite oxidase was purified homogeneously from strain D3-2, the oxidized and reduced forms of the purified sulfite oxidase absorption spectra corresponded to those of A. ferrooxidans aa 3-type cytochrome c oxidase. The confirmed molecular weights of the α-subunit (52.5 kDa), the β-subunit (25 kDa), and the γ-subunit (20 kDa) of the purified sulfite oxidase and the N-terminal amino acid sequences of the γ-subunit of sulfite oxidase (AAKKG) corresponded to those of A. ferrooxidans ATCC 23270 cytochrome c oxidase. The sulfite oxidase activities of the iron- and sulfur-grown A. ferrooxidans D3-2 were much higher than those cytochrome c oxidases purified from A. ferrooxidans strains ATCC 23270, MON-1 and AP19-3. The activities of sulfite oxidase purified from iron- and sulfur-grown strain D3-2 were completely inhibited by an antibody raised against a purified A. ferrooxidans MON-1 aa 3-type cytochrome c oxidase. This is the first report to indicate that aa 3-type cytochrome c oxidase catalyzed sulfite oxidation in A. ferrooxidans.