Monitoring bioremediation in creosote-contaminated soils using chemical analysis and toxicity tests
Monitoring bioremediation in creosote-contaminated soils using chemical analysis and toxicity tests
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DOI：
10.1038/sj.jim.2900789
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发表时间：
2000-02-01
影响因子：
3.4
通讯作者：
Trevors, JT
中科院分区：
文献类型：
--
作者：
Phillips, TM;Liu, D;Trevors, JT
对3种具有杂酚油污染史的土壤（分别为NE、TI和AC）进行了微环境处理，初步研究了促进c -14-芘矿化的条件（养分修正、水分含量和温度）。采用固相Microtox试验、种子萌发和蚯蚓存活试验、sos显色试验、toxi显色试验和红细胞溶血试验监测生物修复情况。150天后AC土壤中的污染物浓度没有变化。根据蚯蚓、种子萌发和Microtox测试，东北土壤中多环芳烃（PAH）浓度下降，毒性总体下降。虽然TI土壤中的总石油烃（TPHs）在处理后减少，但蚯蚓、种子萌发、RBC和Microtox测试的结果表明，毒性最初增加，表明在生物降解过程中可能形成了有毒的中间代谢物。毒性测试结果并不总是与污染物浓度相关，每次测试所显示的趋势也不一致。每项测试都显示出检测土壤污染减少的不同能力。
Three soils with a history of creosote contamination (designated NE, TI and AC) were treated in bench-scale microcosms using conditions (nutrient amendment, moisture content and temperature) which had promoted mineralization of C-14-pyrene in a preliminary study. Bioremediation was monitored using the solid-phase Microtox test, seed germination and earthworm survival assays, SOS-chromotest, Toxi-chromotest and a red blood cell (RBC) haemolysis assay. Contaminant concentrations in the AC soil did not change after 150 days. Polycyclic aromatic hydrocarbon (PAH) concentrations decreased in the NE soil, and toxicity decreased overall according to the earthworm, seed germination and Microtox tests. Although total petroleum hydrocarbons (TPHs) in the TI soil were reduced following treatment, results of the earthworm, seed germination, RBC and Microtox tests suggested an initial increase in toxicity indicating that toxic intermediary metabolites may have formed during biodegradation. Toxicity testing results did not always correlate with contaminant concentrations, nor were the trends indicated by each test consistent for any one soil. Each test demonstrated a different capacity to detect reductions in soil contamination.