Detection of early cartilage damage using targeted nanosomes in a post-traumatic osteoarthritis mouse model
Detection of early cartilage damage using targeted nanosomes in a post-traumatic osteoarthritis mouse model
复制标题
DOI：
10.1016/j.nano.2015.01.011
复制
发表时间：
2015-05-01
影响因子：
5.4
通讯作者：
Hasty, Karen A.
中科院分区：
文献类型：
--
作者：
Cho, Hongsik;Pinkhassik, Eugene;Hasty, Karen A.
骨关节炎（OA）是美国疼痛和残疾的主要原因。早期干预的一个问题是，在不可逆损伤已经发生之前很难发现OA。本研究描述了一种利用荧光纳米体在创伤后骨关节炎（pta）小鼠模型中早期检测OA的新方法。在本研究中，压缩载荷诱导小鼠膝关节损伤。利用包封荧光染料并与II型胶原抗体结合的纳米体检测软骨损伤。通过荧光成像（IVIS）和组织病理学检测软骨损伤和OA进展。组织病理学分析显示发生轻度骨关节炎改变。由于更多的纳米体结合，这与IVIS成像上更高的荧光相对应。这些结果表明，治疗诊断纳米体可能有助于早期pta的检测以及靶向传递介入性药物。来自临床编辑：随着人口老龄化，骨关节炎现在是一个全球性的重大问题。早期发现可能有助于减缓疾病的发展。在这项研究中，作者描述了使用荧光纳米体来检测骨关节炎小鼠模型的早期软骨损伤。这种检测方法也可能被证明是有用的靶向递送药物在未来。Elsevier Inc.出版。
Osteoarthritis (OA) is a major cause of pain and disability in the US. A problem with early intervention is that it is very difficult to detect OA before irreversible damage has already occurred. This study characterizes a novel method of early OA detection in a mouse model of post-traumatic osteoarthritis (PTOA) using fluorescent nanosomes. In this investigation, knee injury was induced in mice by compressive loading. Nanosomes encapsulating fluorescent dye and conjugated to collagen type II antibody were utilized to detect cartilage damage in vivo. Cartilage damage and OA progression were detected by the use of fluorescence-imaging (IVIS) and histopathology. Histopathology analyses showed that mild osteoarthritic changes had occurred. This corresponded with a higher fluorescence on IVIS imaging due to more nanosome binding. These results suggest that theragnostic nanosomes may be useful for detection of early PTOA as well as for targeted delivery of interventional agents.From the Clinical Editor: With the aging population, osteoarthritis now poses a significant problem worldwide. Early detection may help slow the progression of the disease. In this study, the authors described the use of fluorescent nanosomes to detect early cartilage damage in a mouse model of osteoarthritis. This detection method may also prove to be useful for targeted delivery of drugs in the future. Published by Elsevier Inc.