The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells
The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells
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RPL22 siRNA对ET-1诱导的人肺动脉平滑肌细胞增殖的影响
DOI：
10.3892/ijmm.2012.992
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发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
Wang, Hong
中科院分区：
文献类型：
--
作者：
Sun, Kai;Xue, Hong;Wang, Hong
本研究旨在探讨抗核糖体蛋白L22 （RPL22）小干扰RNA （siRNA）对et -1诱导的人肺动脉平滑肌细胞（HPASMCs）增殖的影响。HPASMCs转染抗RPL22的siRNA，在平滑肌细胞培养基（SMCM）和ET-1中孵育。real-time PCR和western blotting分别检测HPASMCs RPL22基因表达和蛋白水平。采用PCNA、CCK-8免疫组织化学和流式细胞术检测HPASMC的增殖情况。同时检测细胞周期蛋白D1 （CCND1）的表达。用si RNA转染RPL22后，对照组和et - 1组的RPL22表达明显受到抑制。转染RPL22的siRNA可显著抑制HPASMC的增殖。此外，CCND1也通过抑制RPL22的表达而下调。综上所述，抑制RPL22的表达可以抑制HPASMC的增殖和CCND1的表达。抗RPL22的siRNA对HPASMC增殖的影响被认为是通过抑制CCND1的表达介导的。
The aim of this study was to investigate the effects of small interference RNA (siRNA) against ribosomal protein L22 (RPL22) on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells (HPASMCs). HPASMCs were transfected with siRNA against RPL22, incubated in smooth muscle cell culture medium (SMCM) and ET-1. RPL22 gene expression and protein levels of HPASMCs were measured by real-time PCR and western blotting, respectively. PCNA, CCK-8 immunohistochemistry and flow cytometry analysis were used to evaluate HPASMC proliferation. Cyclin D1 (CCND1) expression was also assayed. Following transfection with si RNA against RPL22,RPL22 expression was significantly inhibited in the control and ET-I groups. HPASMC proliferation was significantly suppressed by transfection with siRNA against RPL22. Moreover, CCND1 was also downregulated by inhibiting RPL22 expression. In conclusion, these data suggest that inhibition of RPL22 expression can suppress HPASMC proliferation and CCND1 expression. The effects of siRNA against RPL22 on HPASMC proliferation are considered to be mediated through inhibition of CCND1 expression.