Characterization of microsatellite loci in the black rhinoceros (Diceros bicornis) and white rhinoceros (Ceratotherium simum): their use for cross-species amplification and differentiation between the two species
Characterization of microsatellite loci in the black rhinoceros (Diceros bicornis) and white rhinoceros (Ceratotherium simum): their use for cross-species amplification and differentiation between the two species
复制标题
黑犀牛（Diceros bicornis）和白犀牛（Ceratotherium simum）微卫星位点的特征：它们在跨物种扩增和两个物种之间的分化中的应用
DOI：
10.1007/s10592-007-9321-3
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发表时间：
2008
影响因子：
2.2
通讯作者：
A. Alcivar
中科院分区：
文献类型：
--
作者：
L. Nielsen;Dawn Meehan;Annelisa M. Kilbourn;A. Alcivar
随着黑犀牛和白犀牛的种群规模持续下降，需要在多个地区做出更多努力来帮助保护工作。正在探索的一个领域是利用遗传多样性信息来帮助做出保护决策。在本研究中，我们为白犀牛和黑犀牛设计了21个微卫星引物，其中分别扩增了白犀牛和黑犀牛的16个和17个带。在这些引物中，所有 16 个在白犀牛中都是多态性的，17 个引物中的 12 个在黑犀牛中是多态性的。白犀牛和黑犀牛的等位基因平均数为 3.31 和 2.12，预期杂合度为 0.420 和 0.372，观察到的杂合度分别为 0.436 和 0.322。其中七个引物产生了不同的等位基因大小和变异，可以区分黑犀牛和白犀牛。建议对更大的野生种群样本量和标记进行进一步的遗传分析，以更好地了解黑犀牛和白犀牛种群的遗传结构，以便有助于这些极度濒危物种的保护工作。
As the population sizes of the black and white rhinoceroses continues to decline, more efforts are needed in multiple areas to help with the conservation efforts. One area being explored is the use of genetic diversity information to aid conservation decisions. In this study, we designed 21 microsatellite primers for white and black rhinoceroses, 16 and 17 of which amplified bands in the white and black rhinoceros, respectively. Out of these primers all 16 were polymorphic in the white rhinoceros and 12 of the 17 were polymorphic in the black rhinoceros. The mean number of alleles was 3.31 and 2.12, the expected heterozygosities were 0.420 and 0.372, and the observed heterozygosities were 0.436 and 0.322 for the white and black rhinoceroses, respectively. Seven of the primers produced different allele sizes and variations that distinguished between black and white rhinoceroses. Further genetic analyses with larger wild population sample sizes and markers are recommended to obtain a better understanding of the genetic structure of the black and white rhinoceros populations in order to be useful in the conservation efforts of these critically endangered species.