Cross-Species RNA-Seq Study Comparing Transcriptomes of Enriched Osteocyte Populations in the Tibia and Skull.
Cross-Species RNA-Seq Study Comparing Transcriptomes of Enriched Osteocyte Populations in the Tibia and Skull.
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DOI：
10.3389/fendo.2020.581002
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发表时间：
2020
影响因子：
5.2
通讯作者：
Skerry TM
中科院分区：
文献类型：
--
作者：
Wang N;Niger C;Li N;Richards GO;Skerry TM
骨骼不同区域的骨骼之间的局部位点特异性差异解释了它们不同的特性和功能。为了确定这些差异背后的机制，我们进行了一项跨物种研究，比较了颅骨和胫骨骨细胞的RNA转录组，这些骨细胞来自同一只小鼠、大鼠和恒河猴，具有非常不同的主要功能和生理反应。通过生物信息学分析，确定了32个基因在位点之间发生相同方向的变化，并在所有三个物种中共享。在所有三种动物的胫骨中，几个已确定的骨生长和重塑关键基因（BMP7, DKK1, FGF1， FRZB， SOST）均上调。其中许多与Wnt信号通路相关或串扰。这些结果表明，Wnt信号相关的候选物在颅骨和胫骨的骨稳态调节机制中具有不同的控制作用，并表明遗传决定的结构和不同部位机械载荷诱导的应变反馈机制之间存在不同的平衡。
Local site-specific differences between bones in different regions of the skeleton account for their different properties and functions. To identify mechanisms behind these differences, we have performed a cross-species study comparing RNA transcriptomes of cranial and tibial osteocytes, from bones with very different primary functions and physiological responses, collected from the same individual mouse, rat, and rhesus macaque. Bioinformatic analysis was performed to identify 32 genes changed in the same direction between sites and shared across all three species. Several well-established key genes in bone growth and remodeling were upregulated in the tibias of all three species (BMP7, DKK1, FGF1, FRZB, SOST). Many of them associate or crosstalk with the Wnt signaling pathway. These results suggest Wnt signaling-related candidates for different control of regulatory mechanisms in bone homeostasis in the skull and tibia and indicate a different balance between genetically determined structure and feedback mechanisms to strains induced by mechanical loading at the different sites.