The chlorinated AHR ligand 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) promotes reactive oxygen species (ROS) production during embryonic development in the killifish (Fundulus heteroclitus)
The chlorinated AHR ligand 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) promotes reactive oxygen species (ROS) production during embryonic development in the killifish (Fundulus heteroclitus)
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DOI：
10.1016/j.aquatox.2005.07.013
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发表时间：
2006-01-05
影响因子：
4.5
通讯作者：
Elskus, A
中科院分区：
文献类型：
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作者：
Arzuaga, X;Wassenberg, D;Elskus, A
暴露于二恶英类化学物质中，可以激活芳烃受体（AHR），导致细胞和组织活性氧（ROS）的产生增加。在鱼类早期发育过程中，人们对这些影响知之甚少。我们使用了Fundulus heteroclitus鱼模型，以确定AHR配体和促氧化剂3,3',4,4'，5-五氯联苯（PCB126）是否可以在鳉鱼发育过程中增加ROS的产生，并测试一种新的无创测量活体ROS的方法。采用超氧敏感荧光染料双氢乙啶（DHE）对暴露于PCB 126或载体的发育中的鳉鱼进行了卵ROS生成的显微检测。卵内CYP1A活性（乙氧基间苯二酚-o-去乙基化酶，EROD）和卵内ROS均受到PCB126的诱导。在卵细胞中，浓度低至0.003 nM的PCB126可诱导CYP1A活性，在浓度为0.3 nM的PCB126处诱导效果最大。这些PCB 126浓度也显著增加了胚胎肝脏中卵子ROS的产生，早在受精后5天就可以检测到ROS。这些数据表明，促氧化剂和CYP1A诱诱剂PCB126增加了发育中的鳉鱼胚胎中CYP1A的活性和ROS的产生。本文中描述的超氧化物检测试验（SoDA）提供了一种半定量的、易于测量的、改变ROS产生的早期指标，可以与同时在卵中测量CYP1A活性和胚胎发育一起使用，以探索对AHR配体的生化、生理和发育反应之间的功能关系。(c) 2005 Elsevier B.V.版权所有
Exposure to dioxin-like chemicals that activate the aryl hydrocarbon receptor (AHR) can result in increased cellular and tissue production of reactive oxygen species (ROS). Little is known of these effects during early fish development. We used the fish model, Fundulus heteroclitus, to determine if the AHR ligand and pro-oxidant 3,3',4,4',5-pentachlorobiphenyl (PCB126) can increase ROS production during killifish development, and to test a novel method for measuring ROS non-invasively in a living organism. The superoxide-sensitive fluorescent dye, dihydroethidium (DHE), was used to detect in ovo ROS production microscopically in developing killifish exposed to PCB 126 or vehicle. Both in ovo CYP1A activity (ethoxyresorufin-o-deethylase, EROD) and in ovo ROS were induced by PCB126. In ovo, CYP1A activity was inducible by PCB126 concentrations as low as 0.003 nM, with maximal induction occurring at 0.3 nM PCB 126. These PCB 126 concentrations also significantly increased in ovo ROS production in embryonic liver, ROS being detectable as early as 5 days post-fertilization. These data demonstrate that the pro-oxidant and CYP1A inducer, PCB126, increases both CYP1A activity and ROS production in developing killifish embryos. The superoxide detection assay (SoDA) described in this paper provides a semi-quantitative, easily measured, early indicator of altered ROS production that can be used in conjunction with simultaneous in ovo measurements of CYP1A activity and embryo development to explore functional relationships among biochemical, physiological and developmental responses to AHR ligands. (c) 2005 Elsevier B.V. All rights reserved.