Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.
Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.
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自体淋巴细胞治疗实验性犬淋巴水肿：一项试点研究。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Bernard McC. O‘Brien
中科院分区：
文献类型：
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作者：
Kenneth R. Knight;Morris Ritz;D. Lepore;Russell Booth;Keryn Octigan;Bernard McC. O‘Brien
阻塞性淋巴水肿，富含蛋白质的液体在间质空间中的积聚，在5只狗中通过一个腹股沟的照射和随后的任何剩余的淋巴结的手术消融的组合而产生。淋巴水肿稳定长达2年。目的是测试动脉内注射自体淋巴细胞治疗淋巴水肿的疗效。假设是淋巴细胞产生的细胞因子介导淋巴水肿肢体中巨噬细胞蛋白酶的蛋白水解，以去除多余的蛋白质并减轻水肿。通过Ficoll-Paque方法从血液中分离浓缩的富含淋巴细胞的制剂。将这些制剂注射到股动脉中4次，间隔约4周。第一次注射淋巴细胞后三个月，淋巴水肿肢体的平均多余周长与对侧对照肢体相比明显减少了69%。治疗后，皮肤厚度和羟脯氨酸含量（两种纤维化指标）以及含水量（水肿指标）显著降低。与对照组相比，组织液和皮肤匀浆中的酸性蛋白酶活性增加，蛋白浓度显着降低。可以得出结论，增加蛋白水解，可能是由于激活巨噬细胞招募淋巴水肿肢体，可能部分解释这些结果。
Obstructive lymphoedema, an accumulation of protein-rich fluid in interstitial spaces, was created in five dogs by a combination of the irradiation of one groin and subsequent surgical ablation of any remaining lymphatics. The lymphoedema was stable for up to 2 years. The aim was to test the efficacy of intra-arterial injection of autologous lymphocytes as a therapy for lymphoedema. The hypothesis was that cytokines produced by lymphocytes mediate proteolysis by macrophage proteinases in the lymphoedematous limb to remove the excess protein and relieve the oedema. A concentrated lymphocyte-rich preparation was isolated from blood by the Ficoll-Paque method. These preparations were injected into the femoral artery four times at approximately 4 weekly intervals. Three months after the first injection of lymphocytes, lymphoedematous limbs showed a marked 69% reduction in the mean excess circumferences compared with opposite control limbs. After treatment, skin thickness and hydroxyproline content (both measures of fibrosis) as well as water content (a measure of oedema) had reduced significantly. In specimens of interstitial fluid and in skin homogenates acidic proteinase activity increased and the protein concentration decreased significantly compared with controls. It is concluded that increased proteolysis, possibly due to activated macrophages recruited to the lymphoedematous limb, may partly explain these results.
美国病理学家协会主席致辞。
DOI：
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发表时间：
1987
期刊：
The American journal of pathology
影响因子：
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作者：
Cotran,RS
通讯作者：
Cotran,RS