Leader proteinase of the beet yellows closterovirus: mutation analysis of the function in genome amplification.
Leader proteinase of the beet yellows closterovirus: mutation analysis of the function in genome amplification.
复制标题
甜菜黄化线状病毒的前导蛋白酶：基因组扩增功能的突变分析。
DOI：
10.1128/jvi.74.20.9766-9770.2000
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发表时间：
2000
影响因子：
5.4
通讯作者：
Dolja,VV
中科院分区：
文献类型：
--
作者：
Peng,CW;Dolja,VV
甜菜黄化病毒前导蛋白酶（L-Pro）具有蛋白酶C端结构域和非蛋白酶N端结构域。结果发现，虽然L-Pro不是基础水平复制所必需的，但其N-末端结构域的缺失导致RNA积累减少1，000倍。N-末端结构域的突变分析显示其结构灵活性，除了在相应的开放阅读框中的54-密码子长的5′-末端元件，其对于在RNA和蛋白质水平上的有效RNA扩增是关键的。
The beet yellows closterovirus leader proteinase (L-Pro) possesses a C-terminal proteinase domain and a nonproteolytic N-terminal domain. It was found that although L-Pro is not essential for basal-level replication, deletion of its N-terminal domain resulted in a 1,000-fold reduction in RNA accumulation. Mutagenic analysis of the N-terminal domain revealed its structural flexibility except for the 54-codon-long, 5′-terminal element in the corresponding open reading frame that is critical for efficient RNA amplification at both RNA and protein levels.