BIOCHEMICAL BASIS OF HIGH-TEMPERATURE INHIBITION OF ETHYLENE BIOSYNTHESIS IN RIPENING TOMATO FRUITS
BIOCHEMICAL BASIS OF HIGH-TEMPERATURE INHIBITION OF ETHYLENE BIOSYNTHESIS IN RIPENING TOMATO FRUITS
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DOI：
10.1111/j.1399-3054.1988.tb09167.x
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发表时间：
1988-03-01
影响因子：
6.4
通讯作者：
HANDA, AK
中科院分区：
文献类型：
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作者：
BIGGS, MS;WOODSON, WR;HANDA, AK
番茄（Lycopersicon esculentum Mill.）CV. Rutgers）在34.degree. C或更高的温度导致与成熟相关的乙烯产量显著下降。乙烯生物合成的高温抑制与永久性组织损伤无关，因为乙烯生产恢复后，果实转移到允许的温度。从25 ℃转移果实后与乙烯生物合成有关的果皮酶活性的测定从35 ℃到40 ℃的温度变化表明，1-氨基环丙烷-1-羧酸（ACC）合酶（EC 4.4.1.14）活性迅速下降，而乙烯形成酶（EFE）活性缓慢下降。去除高温胁迫导致ACC合酶活性相对于EFE活性更快地恢复。果皮组织中ACC的含量反映了热胁迫前、中、后ACC合成酶的活性变化。恢复乙烯生产转移后的果皮盘从高到允许的温度被抑制在放线菌酮的存在下，表明蛋白质合成的必要性。受伤的番茄果皮组织的乙烯产生的抑制高温成熟相关的乙烯产生的整个水果。
Incubation of fruits of tomato (Lycopersicon esculentum Mill. cv. Rutgers) at 34.degree. C or above resulted in a marked decrease in ripening-associated ethylene production. High temperature inhibition of ethylene biosynthesis was not associated with permanent tissue damage, since ethylene production recovered following transfer of fruits to a permissive temperature. Determination of pericarp enzyme activities involved in ethylene biosynthesis following transfer of fruits from 25.degree. C to 35 or 40.degree.C revealed that 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (EC 4.4.1.14) activity declined rapidly while ethylene forming enzyme (EFE) activity declined slowly. Removal of high temperature stress resulted in more rapid recovery of ACC synthase activity relative to EFE activity. Levels of ACC in pericarp tissue reflected the activity of ACC synthase before, during, and after heat stress. Recovery of ethylene production following transfer of pericarp discs from high to permissive temperature was inhibited in the presence of cycloheximide, indicating the necessity for protein synthesis. Ethylene production by wounded tomato pericarp tissue was not as inhibited by high temperature as ripening-associated ethylene production by whole fruits.