Overexpression of mammalian protein kinase C-zeta does not affect the growth characteristics of NIH 3T3 cells.
Overexpression of mammalian protein kinase C-zeta does not affect the growth characteristics of NIH 3T3 cells.
复制标题
哺乳动物蛋白激酶 C-zeta 的过度表达不会影响 NIH 3T3 细胞的生长特性。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
J. Gutkind
中科院分区：
文献类型：
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作者：
P. Crespo;H. Mischak;J. Gutkind
The structure of protein kinase C zeta (PKC zeta) is unusual with respect to other PKCs, as it lacks the C2 domain and possesses only one zinc finger region. Consequently, PKC zeta can not be activated by diacylglycerol or phorbol esters and is not downregulated by prolonged treatment by phorbol esters nor blocked by commonly utilized PKC inhibitors. In this study, we have explored the idea that PKC zeta might participate in proliferative pathways. Our findings show that marked overexpression of mammalian PKC zeta does not alter the growth characteristics of NIH 3T3 cells, nor induces cellular transformation. Furthermore, mammalian PKC zeta does not potentiate the transforming ability of oncogenes such as ras, sis and the muscarinic receptor m1. In this context, PKC zeta or its dominant negative mutant do not affect MAP kinase activation by oncogenes or growth factors. Taken together, our findings demonstrate that mammalian PKC zeta does not directly participate in signaling pathways involved in oncogenic transformation.
