Glass wool filtration leads to a higher percentage of spermatozoa with intact acrosomes: an ultrastructural analysis.
Glass wool filtration leads to a higher percentage of spermatozoa with intact acrosomes: an ultrastructural analysis.
复制标题
玻璃棉过滤导致具有完整顶体的精子百分比更高：超微结构分析。
作者：
K. Sterzik;M. De Santo;S. Uhlich;F. Gagsteiger;E. Strehler
我们研究了不同精子制备技术对人类精子造成超微结构损伤的可能性。将 20 名正常精子男性的精液分成等份，并通过玻璃棉过滤、Percoll 密度梯度离心和作为对照的简单两步离心程序进行处理。对 20 名测试对象（总共 n = 1200）每人 60 颗精子的评估确保了研究足够多的精子。通过扫描电子显微镜评估超微结构损伤。我们研究了精子制备后顶体的状态，并测量了与对照组相比完整精子结构的百分比。与 Percoll 密度梯度离心相比，玻璃棉过滤产生的完整顶体比例显着增加。然而，两种制剂的结果均明显优于对照。总之，玻璃棉过滤和 Percoll 离心都是积累具有完整顶体的精子的有效技术。由于完整顶体的百分比明显较高，玻璃棉过滤似乎是更合适的方法。 Percoll 离心后精子尾部明显弯曲的意义尚不清楚。
We investigated the possibility of ultrastructural damage to human spermatozoa induced by different sperm preparation techniques. Ejaculates from 20 normozoospermic men were divided into equal aliquots and processed by glass wool filtration, Percoll density gradient centrifugation, and a simple two-step centrifugation procedure which served as a control. The evaluation of 60 spermatozoa from each of 20 test subjects (in all, n = 1200) ensured that a sufficiently large number of spermatozoa were investigated. Ultrastructural damage was assessed by scanning electron microscopy. We investigated the state of the acrosome after sperm preparation and measured the percentage of intact spermatozoal structures compared with that of the control. Compared with Percoll density gradient centrifugation, glass wool filtration yielded a significantly increased proportion of intact acrosomes. However, both preparations gave significantly better results than the control. In conclusion, both glass wool filtration and Percoll centrifugation are efficient techniques for the accumulation of spermatozoa with intact acrosomes. Because of the significantly higher percentage of intact acrosomes, glass wool filtration appears to be the more appropriate method. The significance of the conspicuous bending of sperm tails after Percoll centrifugation is not yet known.
影响因子：
6.1
作者：
ALVAREZ, JG;LASSO, JL;STOREY, BT
通讯作者：
STOREY, BT