Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics
Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics
复制标题
通过基因组重测序和转录组学整合对甘蓝型油菜含油量的四个主要QTL进行精修
DOI：
10.1016/j.cj.2022.01.002
复制
发表时间：
2022-06-01
期刊：
影响因子：
6.6
通讯作者：
Li，Maoteng
中科院分区：
文献类型：
--
作者：
Yan，Shuxiang;Li，Huaixin;Li，Maoteng
油菜（Brassica napus）供应中国大约一半的植物油。增加石油产量和寻找控制作物含油量的基因是研究目标。本研究在KenC-8 × N53-2（KN DH）作图群体中检测到4个主效QTL，分别位于A08、A09、C 03和C 06。对亲本品系进行重测序，以鉴定这四个主要QTL区域中影响含油量的结构变异和候选基因。插入-缺失（InDel）标记被开发并用于缩小区域。研究了位于这些区域的差异表达基因。GO和KEGG分析表明，多个基因与脂质代谢相关。鉴定了几个在N53-2中比在KenC-8中表达更高的转录因子。这些结果揭示了油分含量的遗传控制规律，为高油分品种的选育提供了理论依据。
Rapeseed (Brassica napus) supplies about half of the vegetable oil in China. Increasing oil production and searching for genes that control oil content in the crop are research goals. In our previous studies, four major QTL for oil content located on A08, A09, C03 and C06 in the KenC-8 × N53-2 (KN DH) mapping population were detected. The parental lines were resequenced to identify structural variations and candidate genes affecting oil content in these four major QTL regions. Insertion-deletion (InDel) markers were developed and used to narrow the regions. Differentially expressed genes located in the regions were investigated. GO and KEGG analysis showed that several genes were associated with lipid metabolism. Several transcription factors with higher expression in N53-2 than in KenC-8 were identified. These results shed light on the genetic control of oil content and may be helpful for the development of high-oil-content cultivars.