α-DNA I. Synthesis, characterization by high field 1H-NMR, and base-pairing properties of the unnatural hexadeoxyribonudeotide α-[d(CpCpTpTpCpC)] with its complement β-[d(GpGpApApGpG)]
α-DNA I. Synthesis, characterization by high field 1H-NMR, and base-pairing properties of the unnatural hexadeoxyribonudeotide α-[d(CpCpTpTpCpC)] with its complement β-[d(GpGpApApGpG)]
复制标题
α-DNA I. 非天然六脱氧核糖核苷酸 α-[d(CpCpTpTpCpC)] 与其补体 β-[d(GpGpApApApGpG)] 的合成、高场 1H-NMR 表征以及碱基配对特性
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Lown
中科院分区：
文献类型：
--
作者：
F. Morvan;B. Rayner;J. Imbach;D. Chang;J. Lown
采用磷酸三酯法合成了新型脱氧核糖核苷酸α -[d(CpCpTpTpCpC)]及其补体β -[d(GpGpApApGpG)]。α -六聚体的1H-NMR-NOE检查显示，胞嘧啶和胸腺嘧啶碱基似乎在这条链上采用反构象。此外，胸腺嘧啶部分的脱氧核糖可能采用接近C3'-末端的平均构象，而胞苷呋喃糖基团则接近C2'-末端构象。热熔的高显色性和H2O中碱基配对亚胺质子的检测都为α -d[CCTTCC]及其补体β -d[GGAAGG]在pH 7.1含10mm氯化镁的磷酸钾缓冲液中退火提供了证据。在这些条件下，热熔在38摄氏度开始，在大约45摄氏度完成。NOE实验不允许对这对特定序列的退火极性（预测为平行）做出决定。
The novel deoxyribonucleotide alpha-[d(CpCpTpTpCpC)] and its complement beta-[d(GpGpApApGpG)] were synthesized by the phosphotriester method. 1H-NMR-NOE examination of the alpha-hexamer revealed that the cytosine and thymine bases appear to adopt anti conformations in this strand. In addition the deoxyribose of the thymidine moieties may adopt average conformations approximating to C3'-endo while the cytidine furanose groups are close to C2'-endo conformations. Both hyperchromicity in thermal melting and detection of base paired imino protons in 1H-NMR studies in H2O provide evidence for the annealing of alpha-d[CCTTCC] with its complement beta-d[GGAAGG] in potassium phosphate buffer pH 7.1 containing 10 mM magnesium chloride. Under these conditions thermal melting begins at 38 degrees C and its complete at approximately 45 degrees C. NOE experiments do not permit a decision on the polarity of annealing (predicted to be parallel) for this particular pair of sequences.