Post treatments effect on TiZr nanostructures fabricated via anodizing
Post treatments effect on TiZr nanostructures fabricated via anodizing
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DOI：
10.1016/j.jmrt.2019.09.049
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发表时间：
2019-11-01
影响因子：
6.4
通讯作者：
Schmuki, Patrik
中科院分区：
文献类型：
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作者：
Vardaki, Maria;Mohajernia, Shiva;Schmuki, Patrik
本文的主要重点是通过两步阳极氧化工艺在TiZr衬底上制备纳米管，然后进行退火处理，以及它们对表面和生物相容性的影响。优化了两步阳极氧化工艺，以获得均匀有序的纳米管结构，然后在空气中退火或在Ar/H-2环境中还原。高纵横比的纳米管结构（直径160 nm，长度9.4 μ m）在退火或还原后仍保持原有的形貌。当热处理烧掉氟化物时，他们将管转化为ZrTiO4（在空气中退火）或（Zr0.333Ti0.666）O-2（还原）。纳米结构的表面粗糙度和附着力评估也包括在AFM研究中，并表明这些性能是可以调整的。采用HGF-1牙龈成纤维细胞系和LDH、一氧化氮（NO）和活性氧（ROS）测试，分析细胞对TiZr底物和纳米管涂层的体外反应。对于所研究样品的亲水平衡，正如预期的那样，退火后的亲水平衡下降，还原后的亲水平衡增加，细胞响应变化很小。结果表明，这些涂层与人牙龈成纤维细胞具有生物相容性，可用于生物医学应用，而不会产生任何明显的炎症过程。(C) 2019作者。Elsevier B.V.出版
The primary focus of the paper is the fabrication of nanotubes via a two steps anodizing procedure on a TiZr substrate followed subsequent annealing treatments and their effect on surface and biocompatibility. The two step anodizing process is optimized for obtaining uniform and ordered nanotubular structures, that are then annealed in air or reduced in an Ar/H-2 environment. The high aspect ratio nanotubular structures (diameter 160 nm, length 9.4 mu m) maintained their morphology after annealing or reduction. While the thermal treatments burned-off the fluoride, they converted the tubes to ZrTiO4 (annealing in air) or (Zr0.333Ti0.666)O-2 (reducing). Surface roughness and adhesion forces evaluation of the nanostructures was included in the AFM investigations, and show that these properties can be tuned. In vitro cell response to TiZr substrate and nanotubular coatings were analyzed using HGF-1 gingival fibroblasts cell line and LDH, nitric oxide (NO) and reactive oxygen species (ROS) tests. Regarding the hydrophilic balance of the studied samples, as expected it decreased after annealing and but increased after reduction, with only small change in the cell response. The results suggest that these coatings are biocompatible with human gingival fibroblast and can be used in biomedical applications without generating any considerable inflammatory process. (C) 2019 The Authors. Published by Elsevier B.V.