Masseteric inflammation-induced Fos protein expression in the trigeminal interpolaris/caudalis transition zone: contribution of somatosensory-vagal-adrenal integration
Masseteric inflammation-induced Fos protein expression in the trigeminal interpolaris/caudalis transition zone: contribution of somatosensory-vagal-adrenal integration
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DOI：
10.1016/s0006-8993(99)01913-7
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发表时间：
1999-10-23
期刊：
影响因子：
2.9
通讯作者：
Ren, K
中科院分区：
文献类型：
--
作者：
Imbe, H;Dubner, R;Ren, K
本研究观察了迷走神经切断和肾上腺切除对咬肌炎症后脑干神经元Fos蛋白表达的影响，以区分与伤害性加工相关的Fos激活和与躯体自主神经加工相关的Fos激活。炎症是通过在小皮肤切口（S切口）后，在美索比妥麻醉下将完全弗氏佐剂（CFA）单侧注射到咬肌中诱导的。注射CFA后，在双侧三叉神经脊束核（VSP）、孤束核（NTS）、延髓腹外侧核（VLM）和下内侧橄榄核（IOM）内可见Fos阳性神经元。在水平的三叉神经亚核极间/尾侧（Vi/Vc）过渡区，有一个选择性诱导Fos样免疫反应（LI）的VSP和NTS，当与对照组相比（麻醉或不S-切割）。A. S-切割明显激活了尾侧Vc水平VSP内的大部分Fos-LI。双侧肾上腺切除或单侧迷走神经切断可选择性降低炎症诱导的Vis/Vc过渡区VSP（P < 0.05）和NTS（P < 0.05）Fos-LI，但对Vc尾侧Fos-LI影响较小。这些结果表明，咬肌的炎症，orofacial深部组织的损伤，导致在VSP和NTS的神经元活动的广泛变化，部分取决于肾上腺皮质和迷走神经的完整性。因此，除了躯体组织的伤害性反应，口面深部组织损伤也耦合到躯体内脏和躯体自主神经的处理，有助于中枢神经激活。(C)1999 Elsevier Science B. V.保留所有权利。
The effects of vagotomy and adrenalectomy on the expression of Fos protein in brainstem neurons following the inflammation of masseter muscle were examined in order to differentiate the Fos activation related to nociceptive processing in contrast to that due to somatoautonomic processing. The inflammation was induced by a unilateral injection of complete Freund's adjuvant (CFA) into the masseter muscle under methohexital anesthesia after a small skin-cut (S-cut). After the CFA injection, Fos positive neurons were identified in bilateral spinal trigeminal nucleus (VSP), nucleus tractus solitarius (NTS), ventrolateral medulla (VLM) and inferior medial olivary nucleus (IOM). At the level of the trigeminal subnucleus interpolaris/caudalis (Vi/Vc) transition zone, there was a selective induction of Fos-like immunoreactivity (LI) in the VSP and NTS, when compared to control rats (anesthesia with or without S-cut). A. major portion of the Fos-LI in the VSP at the level of the caudal Vc was apparently activated by S-cut. Bilateral adrenalectomy or a unilateral vagotomy resulted in a selective reduction of inflammation-induced Fos-LI in the VSP at the Vi/Vc transition zone (P < 0.05) and NTS (P < 0.05), but had less effect on Fos-LI in the caudal Vc. These results suggest that the inflammation of the masseter muscle, an injury of orofacial deep tissue, results in a widespread change in neuronal activity in the VSP and NTS that depends in part on the integrity of the adrenal cortex and vagus. Thus, in addition to somatotopically organized nociceptive responses, orofacial deep tissue injury also is coupled to somatovisceral and somatoautonomic processing that contribute to central neural activation. (C) 1999 Elsevier Science B.V. All rights reserved.