Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals
Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals
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DOI：
10.1107/s0907444903021784
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发表时间：
2003-12-01
影响因子：
2.2
通讯作者：
Petsko, GA
中科院分区：
文献类型：
--
作者：
Holyoak, T;Fenn, TD;Petsko, GA
收集高能量同步加速器源的质量数据需要选择一种冷冻保护剂，使蛋白质晶体被冷冻冷却，而不会损坏晶体，并抑制冰的形成。丙二酸钠作为盐生长蛋白质晶体的多功能冷冻保护剂的使用在这里介绍。除了其有用的冷冻保护剂性质之外，丙二酸钠还可以用作通用稳定溶液，其允许从盐生长的晶体的操作、衍生化和配体浸泡，这在晶体母液中可能是不可能的。
Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.