An integrated novel plasma-microalgae approach for landfill leachate treatment using a high-ammonia tolerant strain of Chlorella vulgaris
An integrated novel plasma-microalgae approach for landfill leachate treatment using a high-ammonia tolerant strain of Chlorella vulgaris
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DOI：
10.1016/j.algal.2023.103345
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发表时间：
2023-11
期刊：
影响因子：
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通讯作者：
Aya T. Farag;Thomas D. Holmes;D. J. Gilmour;William B. J. Zimmerman
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文献类型：
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作者：
Aya T. Farag;Thomas D. Holmes;D. J. Gilmour;William B. J. Zimmerman
填埋是世界上最常用的处理城市固体废物的方法。然而，垃圾填埋的一个主要缺点是产生垃圾渗滤液(LL)。LL的治疗方法多种多样，主要可分为生物法和物理化学法，但将这两种方法结合起来会产生更有效的治疗方法。在这项研究中，从垃圾渗滤液处理场分离出一株土生普通小球藻，对其进行了纯化和遗传鉴定，用于渗滤液处理。普通小球藻的一个重复，C.V.M*(CCAP 211/141)，在20%的LL(v/v)中有显著的增长，对氨氮有显著的去除(p<；0.05)。为了证实这些发现，两个普通小球藻的复制(C.V.M*和C.V.N)在20%的LL(v/v)中进一步测试。观察到C.V.M*的生长速度是其同行C.V.N的19倍，氨氮的降解率为75%(从浓度290.73 mg/L开始)。当注射等离子体激活的微泡作为前处理步骤时，这一比例进一步提高。经等离子体前处理后，溶液在3h后脱色，氨氮浓度降低1.9倍。结果表明，与未处理的生物量相比，经等离子体预处理的20g/L的生物量增长最快，最终生物量为0.38g/L，总氨氮的去除提高了79%，pH值显著降低(8.66.67)。两个复制体的全基因组测序显示，它们之间的基因类型存在差异，这可能表明可能存在发达的突变或有性繁殖，这可能提高了复制/菌株C.V.M*在20%稀释的LL中耐受更恶劣条件和更高氨氮浓度的能力。这些结果可能为利用高氨耐受性藻类菌株处理LL铺平道路，如果与等离子体预处理相结合，可能会提供一种有效的、环保的和可持续的LL处理方法。
Landfilling is the most common method worldwide to deal with the produced municipal solid wastes (MSW). Nonetheless, one major drawback of landfilling is the production of landfill leachate (LL). Various methods for LL treatment exist which can mainly be divided into biological and physical-chemical methods, however integrating both approaches result in more effective treatment. In this study, an indigenousChlorella vulgariswas isolated from a landfill leachate treatment site, purified, and genetically identified for leachate treatment purposes. One replicate, C.V.M* (CCAP 211/141), of the testedChlorella vulgarisshowed an outstanding growth in 20 % LL (v/v) with a significant ammonia removal (p<0.05). To confirm these findings, both of theChlorella vulgarisreplicates (C.V.M* and C.V.N) were further tested in 20 % LL (v/v). A dramatic increase in the growth of C.V.M* by 19-fold over its peer C.V.N with a 75 % ammonia removal (starting from concentration 290.73 mg/L) was observed. This percentage is further improved when injecting plasma activated microbubbles as a pre-treatment step. The plasma pre-treatment resulted in LL decolourisation after 3 h and reduced ammonia-N concentration by 1.9-fold. C.V.M* showed the highest growth in 20 % LL pre-treated by plasma compared to the untreated LL with a final biomass yield of 0.38 g/L, increase in the total ammonia-N removal (79 %) and a significant decrease in the pH value (8.6–6.67). Whole genome sequencing for both replicates revealed differences in genotypes between them which might indicate the possibility of a developed mutation or sexual reproduction that might have increased the ability of replicate/strain C.V.M* to tolerate harsher conditions and higher ammonia-N concentrations in 20 % diluted LL. These results might pave the way for a possible powerful candidate in LL treatment using a high-ammonia tolerant algal strain which when coupled with plasma pre-treatment might provide an effective, eco-friendly, and sustainable LL treatment approach.