Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses
Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses
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DOI：
10.1128/jvi.70.7.4220-4227.1996
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发表时间：
1996-07-01
影响因子：
5.4
通讯作者：
Luban, J
中科院分区：
文献类型：
--
作者：
Braaten, D;Franke, EK;Luban, J
人类免疫缺陷病毒1型（HIV-1）Gag多聚蛋白与亲环素A结合，并将这种细胞肽基脯氨酰异构酶掺入病毒体中。通过gag突变或通过环孢菌素A破坏亲环素A的掺入，抑制病毒粒子感染性，表明亲环素A在HIV-1生命周期中起重要作用。使用用于将亲环素A包装到病毒粒子中和用于病毒复制对环孢菌素A的敏感性的测定，以及从代表性病毒分离株编码的Gag残基的比对中收集的信息，我们证明了在灵长类免疫缺陷病毒的五个谱系中，只有HIV-1需要亲环素A进行复制。来自HIV-1 M组的进化枝A、B和D的克隆病毒分离物，以及来自黑猩猩的遗传学相关分离物，都需要亲环素A进行复制。相比之下，两个异常值（组0）HIV-1分离株的复制不受破坏亲环蛋白A掺入病毒体的环孢菌素A浓度的影响，表明这些病毒能够独立于亲环蛋白A复制，这些研究确定了HIV-1 M组和0组之间的第一个表型差异，并与系统发育研究一致，表明两种HIV-1 M组和0组之间存在表型差异。1组通过单独的人畜共患病传播事件引入人群。
The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein binds to cyclophilin A and incorporates this cellular peptidyl prolyl-isomerase into virions. Disruption of cyclophilin A incorporation, either by gag mutations or by cyclosporine A, inhibits virion infectivity, indicating that cyclophilin A plays an essential role in the HIV-1 life cycle, Using assays for packaging of cyclophilin A into virions and for viral replication sensitivity to cyclosporine A, as well as information gleaned from the alignment of Gag residues encoded by representative viral isolates, we demonstrate that of the five lineages of primate immunodeficiency viruses, only HIV-1 requires cyclophilin A for replication, Cloned viral isolates from clades A, B, and D of HIV-1 group M, as well as a phylogenetically related isolate from chimpanzee, all require cyclophilin A for replication. In contrast, the replication of two outlier (group 0) HIV-1 isolates is unaffected by concentrations of cyclosporine A which disrupt cyclophilin A incorporation into virions, indicating that these viruses are capable of replicating independently of cyclophilin A, These studies identify the first phenotypic difference between HIV-1 group M and group 0 and are consistent with phylogenetic studies suggesting that the two HIV-1 groups were introduced into human populations via separate zoonotic transmission events.