Role of nerve growth factor in the adult dorsal root ganglia neuron and its response to injury.
Role of nerve growth factor in the adult dorsal root ganglia neuron and its response to injury.
复制标题
神经生长因子在成人背根神经节神经元中的作用及其对损伤的反应。
DOI：
10.1002/cne.902300110
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
JohnsonJr,EM
中科院分区：
文献类型：
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作者：
Rich,KM;Yip,HK;Osborne,PA;Schmidt,RE;JohnsonJr,EM
本文观察了成年豚鼠背根神经节（DRG）神经元对神经生长因子（NGF）剥夺和轴突切断的反应。通过用NGF活性的标准生物测定法测量针对豚鼠NGF的血清抗体滴度水平来监测通过自身免疫方法的NGF剥夺的成功。通过上级颈神经节（SCG）切片中神经元萎缩和明显细胞丢失的组织学证据以及SCG神经递质合成酶活性水平的显著降低（65-80%）证实了抗体产生NGF剥夺。通过使用自身免疫方法，发现了一种新的豚鼠来源，这种豚鼠持续产生高滴度的交叉反应抗NGF抗体。实验设计用于检查感觉神经元在长期缺乏NGF的情况下对损伤的反应。坐骨神经挤压后98天，第六腰椎DRG中的神经元总数在NGF剥夺和对照神经节之间没有差异。DRG神经元尺寸谱的测量显示，与相应对照组相比，未损伤侧和轴突切断侧的NGF剥夺神经元萎缩的证据。与对照组相比，NGF剥夺组豚鼠未损伤感觉神经元的平均体积减少了27.7%（P <0.05）。通过“夹捏试验”和同位素标记轴突运输研究，神经挤压后6天，NGF剥夺组和对照组感觉神经元的再生程度相同。在坐骨神经挤压后98天，采用各种组织学和形态学技术研究腓肠神经，分析长期再生反应。在光学显微镜和超微结构水平上，对照组和NGF剥夺组豚鼠腓肠神经之间无差异。这些研究表明，与对照组相比，失去NGF的感觉神经元的再生能力未受损。
The response of dorsal root ganglia (DRG) neurons to NGF deprivation and to axotomy was examined in adult guinea pigs. The success of NGF deprivation by means of an autoimmune approach was monitored by the measurement of serum antibody titer levels against guinea pig NGF with the standard bioassay for NGF activity. That the antibody produced NGF deprivation was confirmed by histologic evidence of neuronal atrophy and apparent cell loss in sections of the superior cervical ganglia (SCG) and by marked decreases (65–80%) of SCG neurotransmitter‐synthesizing enzyme activity levels. By using the autoimmune approach a new source of guinea pigs was found which consistently produced high titers of cross‐reacting anti‐NGF antibodies.Experiments were designed to examine the response of the sensory neuron to injury while chronically deprived of NGF. Total neuronal counts in the sixth lumbar DRG 98 days after sciatic nerve crush showed no difference between NGF‐deprived and control ganglia. Measurement of the size spectrum of DRG neurons showed evidence of atrophy of the NGF‐deprived neurons in both the uninjured and axotomized side compared to respective controls. The mean volume of uninjured sensory neurons measured in the NGF‐deprived guinea pigs was decreased 27.7% (P <0.05) compared with that of control guinea pigs.The degree of regeneration 6 days following a nerve crush was the same in NGF‐deprived sensory neurons and in controls when measured by the “pinch test” and by isotope‐labeled axonal transport studies. The long‐term regenerative response was analyzed with various histologic and morphometric techniques studying the sural nerve 98 days after sciatic nerve crush. No differences between control and NGF‐deprived guinea pig sural nerves were noted at the light microscopic and ultrastructural levels. These studies demonstrated an unimpaired regenerative capability of sensory neurons deprived of NGF compared with controls.