Ultrahigh-sensitive wireless QCM with bio-nanocapsules
Ultrahigh-sensitive wireless QCM with bio-nanocapsules
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DOI：
10.1016/j.snb.2019.04.150
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
K. Noi;M. Iijima;M. Iijima;Shun’ichi Kuroda;H. Ogi
中科院分区：
文献类型：
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作者：
K. Noi;M. Iijima;M. Iijima;Shun’ichi Kuroda;H. Ogi
石英晶体微天平（QCM）生物传感器是一种质量敏感型生物传感器，通过对检测抗体进行加权可以提高其检测目标蛋白的灵敏度。在这篇文章中，我们提出了一种在无线高频多通道QCM生物传感器中通过生物纳米胶囊（BNC）进行生物标志物的质量放大检测。BNC的分子量为6.57 MDa，在其表面具有许多免疫球蛋白-G（IgG）Fc结合Z结构域，使其能够以定向固定的方式展示IgG 60抗体。我们发现，通过用交联剂增强Z结构域与抗体的Fc部分之间的键合强度，实现了用QCM对生物标志物的高灵敏度检测。我们使用基频高于54 MHz的多通道无线QCM系统进行C-反应蛋白检测。检测限低于10 pg/mL，证实BNC和无线高频QCM的组合允许生物标志物的高灵敏度检测。
Quartz-crystal-microbalance (QCM) biosensor is a mass-sensitive biosensor, and its sensitivity in detecting target proteins can be improved with weighted detection antibodies. In this communication, we propose a mass-amplified detection of biomarkers by bio-nanocapsules (BNCs) in wireless high-frequency multichannel QCM biosensor. BNC exhibits molecular mass of 6.57 MDa and possesses many immunoglobulin-G (IgG) Fc-binding Z domains on its surface, making it possible to display ∼60 antibodies in an oriented immobilization manner. We find that by reinforcing the bond strength between the Z domain and the Fc part of antibody with the cross-linker, a high sensitive detection of biomarkers with QCM is realized. We used multichanel wireless QCM system with fundamental resonance frequencies above 54 MHz and performed detection of C-reactive protein. The detection limit is lower than 10 pg/mL, confirming that combination of BNC and wireless high-frequency QCM allows high sensitivity detection of biomarkers.