Neuroprotection and protein damage prevention by estradiol replacement in rat hippocampal slices exposed to oxygen-glucose deprivation
Neuroprotection and protein damage prevention by estradiol replacement in rat hippocampal slices exposed to oxygen-glucose deprivation
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DOI：
10.1007/s11064-005-2693-1
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Salbego, C
中科院分区：
文献类型：
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作者：
Cimarosti, H;Siqueira, IR;Salbego, C
在此，我们利用暴露于氧 - 葡萄糖剥夺（OGD）的海马切片，研究了雌二醇替代对卵巢切除雌性大鼠的影响。OGD诱导乳酸脱氢酶（LDH）释放到培养液中，这被视为细胞死亡的一个参数。与溶媒处理组相比，雌二醇处理组的LDH释放显著降低了23%。为了研究雌二醇作用的可能机制，我们研究了氧化应激的一些参数。在溶媒处理组和雌二醇处理组中，OGD分别使自由基产生显著增加34%和16%，尽管在总抗氧化能力方面未观察到显著差异。有趣的是，雌二醇替代阻止了在溶媒处理动物中观察到的由OGD引起的色氨酸和酪氨酸含量的显著降低。我们的研究结果表明，卵巢切除雌性大鼠的雌二醇替代降低了细胞对类缺血性损伤的敏感性，并提示该激素在预防蛋白质损伤方面具有作用。
Here we investigated the effects of estradiol replacement in ovariectomized female rats using hippocampal slices exposed to oxygen-glucose deprivation (OGD). OGD induced lactate dehydrogenase (LDH) release to the incubation medium, what was assumed as a parameter of cellular death. In the estradiol-treated group the LDH release was markedly decreased by 23% as compared to the vehicle-treated group. In attempt to study a possible mechanism by which estradiol acts, we investigated some parameters of oxidative stress. In both vehicle-treated and estradiol-treated groups, OGD significantly increased the free radical production by 34% and 16%, respectively, although no significant differences on total antioxidant capacity were observed. Interestingly, estradiol replacement prevented the significant reduction in tryptophan and tyrosine contents caused by OGD observed in vehicle-treated animals. Our results show that estradiol replacement in ovariectomized female rats decreases cellular susceptibility to an ischemic-like injury and suggest a role for the hormone on protein damage prevention.