Association between blood miR-26a levels following artificial insemination, and pregnancy outcome in dairy cattle.
Association between blood miR-26a levels following artificial insemination, and pregnancy outcome in dairy cattle.
复制标题
奶牛人工授精后血液miR-26 a水平与妊娠结局之间的相关性
DOI：
10.1371/journal.pone.0289342
复制
发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
早期妊娠诊断是最大限度提高奶牛群生产力的关键。我们之前的研究表明，在小母牛怀孕的第8天就可以检测到miR-26水平的增加。本研究的目的是确定1)与妊娠失败的奶牛相比，妊娠成功的奶牛在人工授精后早期血浆miR-26水平是否明显升高；2)妊娠成功的奶牛的miRNA水平的早期升高可以通过白细胞（wbc） miRNA表达的变化来解释，可能是由胚胎源性干扰素tau （IFNt）的作用诱导的。3)血浆miRNA水平可提供可靠的妊娠早期预测指标，可用于畜群水平。选取34头泌乳期为1 ~ 4天的奶牛，分别于发情前（D0）和人工授精后9、18 d采集血液，于D32行超声确认妊娠状态。此外，用重组羊IFNt （0-100 pg/ml）体外刺激非妊娠奶牛（n = 4）的白细胞。mirna和ISG15（一种已知的ifnt诱导基因）的水平通过qPCR进行定量。相对于D0，在D9，血浆miR-26a在后来确认怀孕的奶牛（n = 12）中比在怀孕失败的奶牛（n = 22）中增加更多（P = 0.04）。miR-26a在白细胞中的表达不受妊娠状态或体外IFNt刺激的影响（P< 0.01），而ISG15的表达在D18从后来证实怀孕的动物身上收集的白细胞样本中以及体外IFNt刺激后的白细胞中均显著升高（P<0.0001）。最后，ROC分析显示，D9或D18上的miR-26a预测妊娠结局的准确度远低于D18上的WBC ISG15（似然比，2.3 vs 15.4）。总之，成熟奶牛妊娠早期血浆miR-26a水平适度升高，这可能与白细胞中miRNA水平的变化或IFNt的影响无关。此外，与ISG15相比，miR-26a水平的变化可能无法为奶牛妊娠的早期诊断提供准确的测试。
Early pregnancy diagnosis is key to maximise productivity of dairy herds. We previously showed that an increase in the levels of miR-26 could be detected as soon as day 8 of pregnancy in heifers. The aims of this study were to determine whether 1) plasma miR-26 levels would be distinctly elevated, retrospectively, early after artificial insemination in lactating cows with successful compared to failed pregnancies, 2) the early increase in miRNA levels in cows with successful pregnancy could be accounted for by changes in miRNA expression in white blood cells (WBCs), presumably induced by the effects of embryo-derived interferon tau (IFNt), and 3) plasma miRNA levels may provide a reliable early predictor of pregnancy that could be used at a herd level. Blood samples were taken from a total of 34 dairy cows (lactation number 1 to 4) before (D0) and 9 and 18 days after artificial insemination at oestrus, followed by confirmation of pregnancy status by ultrasound on D32. In addition, WBCs collected from non-pregnant cows (n = 4) were stimulated in vitro with recombinant ovine IFNt (0–100 pg/ml). Levels of miRNAs and ISG15, a known IFNt-induced gene, were quantified by qPCR. Relative to D0, a larger increase in plasma miR-26a (P = 0.04) occurred on D9 in cows later confirmed to be pregnant (n = 12) than in cows with a failed pregnancy (n = 22). Expression of miR-26a in WBCs was not affected (P>0.1) by pregnancy status or IFNt stimulation in vitro, in contrast to ISG15 expression which increased markedly (P<0.0001) both in WBC samples collected on D18 from animals later confirmed to be pregnant, and in WBCs after stimulation with IFNt in vitro. Finally, ROC analyses revealed that miR-26a on D9 or D18 could predict pregnancy outcome with much lower accuracy than WBC ISG15 on D18 (Likelihood ratio, 2.3 vs 15.4). In summary, a modest increase in plasma miR-26a levels occurs during early pregnancy in mature dairy cows which may not accounted for by changes in miRNA levels in WBCs or the effects of IFNt. Moreover, compared to ISG15, changes in miR-26a levels may not provide an accurate test for early diagnosis of pregnancy in cows.
登录
查看更多内容
影响因子：
2.5
作者：
Pohler KG;Green JA;Moley LA;Gunewardena S;Hung WT;Payton RR;Hong X;Christenson LK;Geary TW;Smith MF
通讯作者：
Smith MF
DOI：
10.1261/rna.079436.122
发表时间：
2022-10-27
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Ioannidis J;Donadeu FX
通讯作者：
Donadeu FX
影响因子：
3.6
作者：
Krawczynski, Kamil;Najmula, Joanna;Kaczmarek, Monika M.
通讯作者：
Kaczmarek, Monika M.
影响因子：
4.6
作者：
Coutinho, Luiz L.;Matukumalli, Lakshmi K.;Smith, Timothy P. L.
通讯作者：
Smith, Timothy P. L.