RNA polymerase of influenza virus. I. Comparison of the virion-associated RNA polymerase activity of various strains of influenza virus.
RNA polymerase of influenza virus. I. Comparison of the virion-associated RNA polymerase activity of various strains of influenza virus.
复制标题
流感病毒的RNA聚合酶。
DOI：
10.1093/oxfordjournals.jbchem.a133374
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
M. Hamaguchi
中科院分区：
文献类型：
--
作者：
K. Kawakami;A. Ishihama;M. Hamaguchi
对甲型流感病毒4株和2株病毒粒子的多肽组成和RNA聚合酶活性进行了系统的比较研究。变性条件下用聚丙烯酰胺凝胶电泳法测定，不仅血凝素(HA)的相对分子质量有显著差异，核蛋白(NP)和膜蛋白(M)的相对分子质量也有显著差异。结果表明，在由表面蛋白HA和NA(神经氨酸酶)确定的相同血清型的病毒中，病毒蛋白包括内部蛋白的结构存在相当大的差异。在相似条件下生长的不同菌株之间病毒蛋白的相对含量也不同。三种P蛋白的总含量在病毒总蛋白的1.1%到2.2%之间，与病毒粒子相关的RNA聚合酶的活性大致平行。所有受试毒株的病毒粒子相关RNA聚合酶都被相同的二核苷酸引物APG或GPG激活，表明转录启动的特异性在流感病毒的广泛变异中是保守的。
A systematic and comparative study was performed on the polypeptide composition and the RNA polymerase activity associated with virions of various strains of influenza A virus, including four human and two avian viruses. Significant differences were found in the molecular weights of not only hemagglutinin (HA) but also both nucleoprotein (NP) and membrane protein (M), as determined by polyacrylamide gel electrophoresis under denaturing conditions. The results indicate that, among viruses sharing the same serotype determined by the surface proteins HA and NA (neuraminidase), considerable variations exist in the structure of viral proteins, including inner proteins. The relative contents of viral proteins also varied among these strains grown under similar conditions. The total content of three P proteins, the putative RNA polymerase subunits, was within the range between 1.1 and 2.2% of total viral proteins and roughly paralleled the virion-associated RNA polymerase activity. The virion-associated RNA polymerase of all the strains tested were stimulated by the same dinucleotide primers, ApG or GpG, indicating that the specificity of transcription initiation is conserved among wide varieties of influenza virus.