Effect of alginate composition and purity on alginate microspheres
Effect of alginate composition and purity on alginate microspheres
复制标题
DOI：
10.1080/02652040400015452
复制
发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Opara, EC
中科院分区：
文献类型：
--
作者：
Kendall, WF;Darrabie, MD;Opara, EC
Background: Alginate is commonly used to microencapsulate islets in experiments with islet allografts and xenografts for the treatment of Type I diabetes. The purpose of the present study is to determine the effects of alginate composition and purity oil the morphology and size of microspheres. Methods:: Microcapsules produced with the impure alginate types, medium-viscosity high-guluronic acid (IMVG), low-viscosity high-G (ILVG), low-viscosity high-mannuronic acid (ILVM) and medium-viscosity high-M (IMVM) were compared with one another and others generated with a highly purified LVM (HPLVM) alginate. Droplets of 1.5% alginate from an air-syringe pump were gelled in 1.1% CaCl2 solution. While leaving the alginate pressure and needle recess constant, the air-jacket pressure was varied between 93-10.5 PPSI to enhance stable microcapsule generation and different batches of microbeads were made from each alginate type. Results: The sizes of the high-guluronic acid alginate microbeads were consistently bigger than those of the corresponding high-mannuronic acid alginate beads at all air-jacket settings. At the optimal air-jacket pressure of 9.0 PPSI, the mean +/- SD diameter of the IMVG microbeads was 780+20 mum, while that of IMVM was 607+44 mum (p
