A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli
A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli
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DOI：
10.1016/s0167-7012(03)00194-5
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发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Irwin, PL
中科院分区：
文献类型：
--
作者：
Chen, CY;Nace, GW;Irwin, PL
使用 96 孔板和多通道移液器进行连续稀释，然后以 6 x 6 格式在琼脂上滴镀，制定了方案。该方案允许同时接种六种稀释液，这大大减少了所用的平板数量，从而为需要独特环境条件的弯曲杆菌等生物体节省了培养箱空间。由 Elsevier B.V. 出版
A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions. Published by Elsevier B.V.