Pu-erh Tea Regulates Fatty Acid Metabolism in Mice Under High-Fat Diet
Pu-erh Tea Regulates Fatty Acid Metabolism in Mice Under High-Fat Diet
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普洱茶调节高脂饮食小鼠脂肪酸代谢
DOI：
10.3389/fphar.2019.00063
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发表时间：
2019-02-05
影响因子：
5.6
通讯作者：
Jia, Wei
中科院分区：
文献类型：
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作者：
Huang, Fengjie;Wang, Shouli;Jia, Wei
普洱茶具有降血脂作用，但其机制尚不清楚。游离脂肪酸（FFA）通常与肥胖的发展相关，导致2型糖尿病和心血管疾病的风险增加。为了研究普洱茶治疗是否改变FA代谢，我们用普洱茶治疗HFD诱导的肥胖小鼠22周，并使用UPLC-QTOF-MS平台分析实验小鼠的FFA谱。结果表明，普洱茶对小鼠代谢表型和游离脂肪酸组成有显著影响。HFD诱导小鼠明显的肥胖表型，表现为体重、肝脏和脂肪组织重量、血清和肝脏中的脂质水平显著增加，而普洱茶处理显著降低这些参数。在HFD诱导的肥胖小鼠中，几种FFA或FFA比率，如DGLA、棕榈油酸和OA/SA比率显著增加，而SA/PA和AA/DGLA水平显著降低。有趣的是，这些不同的FFA或FFA比率先前被确定为人类肥胖受试者的关键标志物，并且在HFD组中观察到的变化被普洱茶治疗逆转。此外，一组FFA标记物，包括C20：3 n6/C18：3 n6和C20：3 n6/C20：2 n6、C18：3 n6/C18：2 n6、C18：3 n3/C18：2 n6和C24：1 n9/C22：1 n9，这些标记物先前被鉴定为预测接受代谢手术程序的人类受试者中肥胖和糖尿病缓解的生物标志物，通过普洱茶干预被逆转。与HFD组相比，普洱茶显着改善了葡萄糖稳态和胰岛素耐受性。此外，普洱茶处理显著降低了FFA合成基因，并增加了参与FFA摄取和β-氧化的基因的表达，包括FATP 2，FATP 5，PPAR α，CPT 1 α和ACOX-1。这些发现证实了普洱茶对调节脂质和葡萄糖代谢的有益作用，并进一步验证了一组FFA标记物对肥胖和糖尿病的诊断和预后价值。
Pu-erh tea has been extensively reported to possess lipid lowering effects but the underlying mechanisms remained unclear. Free fatty acids (FFAs) are generally correlated with the development of obesity, leading to increased risk for type 2 diabetes mellitus and cardiovascular diseases. To investigate whether Pu-erh tea treatment alters FA metabolism, we treated HFD induced obese mice with Pu-erh tea for 22 weeks and analyzed FFA profiles of experimental mice using a UPLC-QTOF-MS platform. Results showed remarkable changes in metabolic phenotypes and FFA compositions in mice treated with or without Pu-erh tea. HFD induced a marked obese phenotype in mice as revealed by significantly increased body weight, liver and adipose tissue weight, lipid levels in serum and liver, and these parameters were markedly reduced by Pu-erh tea treatment. Several FFA or FFA ratios, such as DGLA, palmitoleic acid, and OA/SA ratio, were significantly increased while the levels of SA/PA and AA/DGLA were significantly reduced in HFD-induced obese mice. Interestingly, these differential FFAs or FFA ratios were previous identified as key markers in human obese subjects, and their changes observed in the HFD group were reversed by Pu-erh tea treatment. Moreover, a panel of FFA markers including C20:3 n6/C18:3 n6 and C20: 3n6/C20: 2 n6, C18:3 n6/C18: 2 n6, C18: 3 n3/C18:2 n6 and C24:1 n9/C22:1 n9, which were previously identified as biomarkers in predicting the remission of obesity and diabetes in human subjects who underwent metabolic surgery procedures, were reversed by Pu-erh tea intervention. Puerh tea significantly improved glucose homeostasis and insulin tolerance compared to the HFD group. Additionally, Pu-erh tea treatment significantly decreased FFA synthesis genes and increased the expression of genes involved in FFA uptake and beta-oxidation including FATP2, FATP5, PPAR alpha, CPT1 alpha, and ACOX-1. These finding confirmed the beneficial effects of Pu-erh tea on regulating lipid and glucose metabolism, and further validated a panel of FFA markers with diagnostic and prognostic value for obesity and diabetes.