Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach
Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach
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DOI：
10.1016/j.dental.2005.08.003
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发表时间：
2006-07-01
期刊：
影响因子：
5
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Takahashi, Yusuke;Imazato, Satoshi;Tay, Franklin R.
目的：由于非创伤性修复治疗（ART）涉及用手动器械去除龋损，因此应考虑改进填充材料以确保更大的成功率。本研究旨在评估含氯己定（CHX）的玻璃离子水门汀（GIC）的抗菌、物理和粘结性能，并确定掺入试剂以获得用于ART方法的抗菌GIC的最佳浓度。将与CHX二盐酸盐组合的CHX二乙酸盐加入到对照GIC粉末中，以获得1/0、2/0、3/0、1/1或2/2%w/w的浓度比。采用琼脂扩散法检测了每种骨水泥对变形链球菌、干酪乳杆菌和内氏放线菌的抗菌活性，并采用HPLC法分析了CHX的释放。结果：所有实验GICs对3种细菌均有抑制作用，但抑菌圈大小和释放的CHX浓度与CHX含量无关。加入2%或更高的CHX二乙酸酯，显著降低抗压强度，并且加入2%或更高的CHX二乙酸酯对牙本质的粘结强度产生不利影响（p < 0.05，ANOVA，Fisher’s PLSD检验），尽管通过添加任何浓度的CHX使凝固时间稍微延长。目前的结果表明，实验GIC含有CHX是有效的，在抑制与龋齿相关的细菌，并掺入1%的CHX二乙酸酯是最佳的，以提供适当的物理和粘结性能。(c)2005年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objectives: Since atraumatic restorative treatment (ART) involves removal of carious lesions with manual instruments, improvement of filling materials to guarantee greater success should be considered. This study aimed to evaluate antibacterial, physical, and bonding properties of glass-ionomer cements (GIC) containing chlorhexidine (CHX), and to determine optimal concentrations for incorporation of agents to obtain antibacterial GICs for use with the ART approach.Methods: CHX diacetate combined with CHX dihydrochloride was added to control GIC powder to obtain concentration ratios of 1/0, 2/0, 3/0, 1/1, or 2/2% w/w. Antibacterial activity of each cement against Streptococcus mutans, Lactobacillus casei or Actinomyces naeslundii was examined using agar-diffusion methods, and release of CHX was analyzed by HPLC. Compressive strength, bond strength to dentin, and setting time were measured, and compared with those of control samples.Results: All experimental GICs exhibited inhibition of three bacteria, but sizes of inhibition zones and concentrations of CHX released were not dependent upon CHX content. Incorporation of CHX diacetate at 2% or greater, significantly decreased compressive strength, and bond strength to dentin was adversely affected by addition of CHX diacetate at 2% or more (p < 0.05, ANOVA, Fisher's PLSD test), although setting time was extended a little by addition of any concentrations of CHX.Significance: The present results demonstrate that experimental GICs containing CHX are effective in inhibiting bacteria associated with caries, and incorporation of 1% CHX diacetate is optimal to give appropriate physical and bonding properties. (c) 2005 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.