Electrophoretic Mobilities of the Charge Variants of DNA and Other Polyelectrolytes: Similarities, Differences, and Comparison with Theory.
Electrophoretic Mobilities of the Charge Variants of DNA and Other Polyelectrolytes: Similarities, Differences, and Comparison with Theory.
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DNA 和其他聚电解质的电荷变体的电泳迁移率：相似点、差异以及与理论的比较。
DOI：
10.1021/acs.jpcb.6b10599
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Stellwagen,NancyC
中科院分区：
文献类型：
--
作者：
Stellwagen,NancyC
自由溶液的电泳迁移率与不同的电荷密度的聚电解质进行了分析，使用的数据取自文献。聚离子包括单链和双链DNA低聚物、小芳香分子、肽、蛋白质和合成共聚物。通过计算迁移率比，将每个数据集中每个电荷变体的迁移率除以该数据集中最高电荷聚离子的迁移率，使由于背景电解质差异引起的迁移率变化最小化。在所有情况下，迁移率比与分数电荷的对数线性增加，而不是通常假设的电荷的一次幂。此外，所有聚电解质，除了合成的共聚物，所观察到的迁移率比表现出共同的依赖分数电荷的对数。对于合成共聚物观察到的独特结果可能是由于它们的烃主链的柔性，与其它聚电解质的相对刚性的亲水性主链相反。观察到的DNA电荷变体的迁移率预测的曼宁电泳方程，而zeta电位理论预测的迁移率较高。然而，从这两个理论计算的迁移率比与观测结果一致。
Free solution electrophoretic mobilities of polyelectrolytes with different charge densities have been analyzed using data taken from the literature. The polyions include single- and double-stranded DNA oligomers, small aromatic molecules, peptides, proteins, and synthetic copolymers. Mobility variations due to differences in the background electrolytes were minimized by calculating mobility ratios, dividing the mobility of each charge variant in each data set by the mobility of the most highly charged polyion in that data set. In all cases, the mobility ratios increase linearly with the logarithm of the fractional charge, not the first power of the charge as usually assumed. In addition, the mobility ratios observed for all polyelectrolytes, except for the synthetic copolymers, exhibit a common dependence on the logarithm of fractional charge. The unique results observed for the synthetic copolymers may be due to the flexibility of their hydrocarbon backbones, in contrast to the relatively rigid hydrophilic backbones of the other polyelectrolytes. The mobilities observed for the DNA charge variants are well predicted by the Manning electrophoresis equation, whereas the mobilities predicted by zeta potential theories are higher. However, mobility ratios calculated from both theories agree with the observed results.