DISTRIBUTION OF GLUCOSE AND LACTATE IN THE INTERPHOTORECEPTOR MATRIX
DISTRIBUTION OF GLUCOSE AND LACTATE IN THE INTERPHOTORECEPTOR MATRIX
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DOI：
10.1159/000267174
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发表时间：
1992-07-01
影响因子：
2.1
通讯作者：
SOUTHWICK, RE
中科院分区：
文献类型：
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作者：
ADLER, AJ;SOUTHWICK, RE
视网膜的感光细胞从视网膜后面的脉络膜血液供应获得营养。如果细胞外感光细胞间基质（IPM）介导这种视网膜营养，那么营养物质（如葡萄糖）和废物（如乳酸）的浓度梯度预计将跨越IPM，从神经视网膜到视网膜色素上皮细胞（RPE）;这一假设在这里进行检查。采用温和的方法从牛眼的视网膜表面（IPM-R）和根尖RPE表面（IPM-P）制备IPM，邻近组织的污染非常低。IPM-R（180穆尔）和IPM-P（87穆尔）隔室的实际固有体积由加入到用于样品制备的洗涤缓冲液中的台盼蓝染料的稀释液估算;这些测量值是计算浓度所需的。在IPM-R样品中，葡萄糖几乎检测不到，乳酸盐浓度非常高（13 mM）。在RPE表面（IPM-P）附近，两种化合物的存在水平（葡萄糖0.9 mM，乳酸盐3.8 mM）与血清中的水平更接近。用新鲜兔眼获得了类似的结果。这种分布差异（营养物更集中在RPE附近，废物靠近视网膜）表明梯度与IPM作为视网膜外营养途径的利用一致。
The photoreceptor cells of the retina derive their nourishment from the choroidal blood supply behind the retina. If the extracellular interphotoreceptor matrix (IPM) mediates this retinal nutrition, then gradients in concentrations of nutrients such as glucose and waste products such as lactic acid would be expected across the IPM, from the neural retina to the retinal pigment epithelium (RPE); this hypothesis is examined here. Gentle methods were employed to prepare IPM from the retinal surface (IPM-R) and apical RPE surface (IPM-P) of bovine eyes, and contamination from adjacent tissues was very low. The actual intrinsic volumes of the IPM-R (180 mul) and IPM-P (87 mul) compartments were estimated from dilution of trypan blue dye added to the wash buffer used for sample preparation; these measurements were required for calculation of concentrations. In the IPM-R samples glucose was virtually undetectable and the lactate concentration was very high (13 mM). Near the RPE surface (IPM-P) both compounds were present at levels (glucose 0.9 mM, lactate 3.8 mM) more comparable to those in blood serum. Similar results were obtained with fresh rabbit eyes. This difference in distribution (nutrient more concentrated near RPE, waste product near retina) indicates gradients consistent with the utilization of the IPM as a pathway for outer-retinal nutrition.