Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG)
Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG)
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DOI：
10.1016/s0165-5728(99)00010-7
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发表时间：
1999-06-01
影响因子：
3.3
通讯作者：
Linington, C
中科院分区：
文献类型：
--
作者：
Brehm, U;Piddlesden, SJ;Linington, C
We describe the epitope specificity of a panel of ten demyelinating monoclonal antibodies (mAb) that recognise the extracellular immunoglobulin-like domain of human myelin oligodendrocyte glycoprotein (hMOG(Igd)). All the mAbs bind to the surface of MOG-transfected fibroblasts as assessed in vitro by FAGS and immunocytochemistry but failed to recognise overlapping 15-mer MOG peptides when assessed by ELISA. However, increasing peptide length to 25 amino acids revealed that four mAbs recognised epitopes within the amino acid sequence 63-100 of human MOG. In contrast, a non-demyelinating MOG-specific mAb recognised MOG by both ELISA and Western blotting but failed to stain MOG transfected fibroblasts. These observations suggest that assays based on the use of MOG-transfected cell lines will differentiate between pathogenic and non-pathogenic MOG-specific antibody responses in experimental models and human diseases of the nervous system. (C) 1999 Elsevier Science B.V. All rights reserved.
