CIP2A is Overexpressed in Non-Small Cell Lung Cancer and Correlates with Poor Prognosis
CIP2A is Overexpressed in Non-Small Cell Lung Cancer and Correlates with Poor Prognosis
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DOI：
10.1245/s10434-010-1313-8
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发表时间：
2011-03-01
影响因子：
3.7
通讯作者：
Wang, En-Hua
中科院分区：
文献类型：
--
作者：
Dong, Qian-Ze;Wang, Yang;Wang, En-Hua
蛋白磷酸酶癌抑制剂2A（CIP 2A）是一种抑制c-MYC蛋白水解降解的癌蛋白。本研究采用定量逆转录聚合酶链反应（RT-QPCR）检测了29例NSCLC组织中CIP 2A mRNA的表达，并分析了CIP 2A在NSCLC中的临床意义。我们还通过免疫组化检测了90例肺癌标本中CIP 2A蛋白的表达，并将其表达与c-MYC表达和临床病理参数进行了相关性分析。在29例肺癌组织中，有24例（82.7%）的肺癌组织中CIP 2A mRNA的表达水平明显高于相应的正常组织。此外，CIP 2A mRNA表达水平与c-MYC mRNA水平相关。在90例肺癌组织中，CIP 2A蛋白表达阳性率为72.2%，与肺癌患者的生存率相关（P < 0.05）。此外，CIP 2A状态是NSCLC患者的重要预后因素（P = 0.0136）。CIP 2A是一种在非小细胞肺癌中过表达的癌蛋白，其表达与预后不良和恶性细胞增殖有关。
Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein inhibiting proteolytic degradation of c-MYC. In this study, we investigated the clinical relevance of CIP2A in NSCLC.We analyzed CIP2A mRNA expression in 29 NSCLC tissues using quantitative reverse transcription polymerase chain reaction (RT-QPCR). We also examined the expression of CIP2A protein by immunohistochemistry in 90 lung cancer specimens and correlated its expression with c-MYC expression and clinicopathological parameters. The functional roles of CIP2A in lung cancer cell lines were evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation and invasion.In 29 lung cancer tissues examined, 24 of them (82.7%) exhibited much stronger levels of CIP2A mRNA compared with their corresponding normal tissues. Moreover, CIP2A mRNA expression levels correlated with c-MYC mRNA levels. Furthermore, CIP2A protein was found to be overexpressed in 72.2% of 90 human lung cancer samples and correlated with poor survival (P < 0.05). In addition, the CIP2A status was a significant prognostic factor for NSCLC patients (P = 0.0136). Depleting CIP2A expression inhibited growth and clonogenic potential in lung cancer cell lines.CIP2A is an oncoprotein overexpressed in NSCLC, and its expression is associated with poor prognosis and malignant cell proliferation.