Association between cigarette smoking and release of tumour necrosis factor α and its soluble receptors by peripheral blood mononuclear cells in patients with rheumatoid arthritis
Association between cigarette smoking and release of tumour necrosis factor α and its soluble receptors by peripheral blood mononuclear cells in patients with rheumatoid arthritis
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DOI：
10.1093/rheumatology/kel094
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发表时间：
2006-10-01
期刊：
影响因子：
5.5
通讯作者：
Mattey, D. L.
中科院分区：
文献类型：
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作者：
Glossop, J. R.;Dawes, P. T.;Mattey, D. L.
目标。目的探讨吸烟与类风湿关节炎患者外周血单个核细胞（PBMCs）释放tnf - α及其可溶性受体（sTNFRI和sTNFRII）的关系。我们研究了71例已确诊的RA患者（平均病程10.6年）。通过问卷调查确定吸烟史。从外周血中分离T淋巴细胞和单核细胞，分别用植物血凝素和脂多糖刺激或不刺激孵育。采用酶联免疫吸附法测定培养液中tnf - α和sTNFR的释放。有吸烟史患者受刺激T淋巴细胞tnf - α释放明显高于无吸烟史患者（1416.0 pg/ml vs 767.4 pg/ml， P=0.04），且与吸烟持续时间和吸烟强度有关（P为趋势）
Objective. To investigate the relationship between cigarette smoking and release of TNF-alpha and its soluble receptors (sTNFRI and sTNFRII) by peripheral blood mononuclear cells (PBMCs) from RA patients.Methods. We studied 71 RA patients with established disease (mean duration 10.6 yr). Smoking history was established by questionnaire. T lymphocytes and monocytes were isolated from peripheral blood and incubated with or without stimulation (phytohaemagglutinin and lipopolysaccharide, respectively). Release of TNF-alpha and sTNFR into culture medium was measured by enzyme-linked immunosorbent assay.Results. TNF-alpha release by stimulated T lymphocytes was significantly higher in patients with a history of smoking than in those who had never smoked (1416.0 vs 767.4 pg/ml, P=0.04), and showed a relationship with smoking duration and intensity (P for trend