The locus of initial calcification in cartilage and bone.
The locus of initial calcification in cartilage and bone.
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DOI：
10.1097/00003086-197107000-00010
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发表时间：
1971-01-01
影响因子：
4.2
通讯作者：
Bonucci, E
中科院分区：
文献类型：
--
作者：
Bonucci, E
初始钙化的位置可定义为有机大分子的折叠，它诱导并定位于钙化。显微镜，特别是电子显微镜，为尽可能准确地分析这种支架的性质和结构提供了有价值的证据。因此，下面的大部分讨论主要是基于超微结构的发现。迄今为止积累的数据未能在一群相互竞争的理论之间继续发生冲突。原因不难找到。争论往往导致研究集中在一个有限的部门，而最后一系列的相关现象没有得到注意;和各种技术困难，涉及准备矿化组织的电子显微镜有不同的结果。一些特殊的危险，如由于在高跷溶液中固定而引起的沉淀或成核现象，”切片过程中切片被水钙化“，”电子染色“引起的磷灰石晶体精细结构的变化，以及由于电子束 * 3而引起的矿物升华和再沉淀，
The locus of initial calcification can be de-fincd as the scalfolding of organic macro-moleculcs which induce and localize mincr-ulization. The microscope—particularly the clectron microscope-yields valuable cvidence in tlıc quest to analyze the propertics and structure of this scaffolding as accurately as possible. Most of the discussion which follows is, therefore, based primarily on ultrastructural findings.'l'hc data so far accumulated havc failcd to still continual skirmishes between a bevy of competing theories. Thc reasons arc not hard to find. Controversy has often led to research focuscul on onc limited sector, whilc'ast ranges of relevant phenomena havc escaped attention; and various technical difficulties involved in preparing mineralized tissues for the clectron microscope have lcd to divergent results. Specific dangers, such s precipitition or nucleation artcfacts duc to fixation in stilt solution," º dccalcifica-tion of uitrithin sections by water during sectioning," changes in fine structurc of tpatite crystıls through “elcctron stains," and mineral sublimation and reprecipitation duc to the clectron beam* 3 have sonnetimes