Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering.
Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering.
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DOI：
10.1016/j.chroma.2006.07.049
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发表时间：
2006-10
期刊：
影响因子：
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通讯作者：
Heejeong Lee;Il-Hwan Cho;M. Moon
中科院分区：
文献类型：
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作者：
Heejeong Lee;Il-Hwan Cho;M. Moon
采用在线流场-流分级（FlFFF）和多角度光散射（MALS）技术，研究了不同溶解温度条件下超分子量（105- 108 g/mol）的羟丙磺酸钠的分子量分布和结构变形。已经通过在三种不同温度条件（5°C、50°C和90°C）下将来自肉瘤液的透明质酸钠（NaHA）材料溶解在水中来研究它们。采用熔块入口非对称流场流分级（FI-AFlFFF）技术，通过现场编程实现了NaHA的分子量分离，并利用MALS在线观察了分级后的NaHA的光散射，确定了分子量分布和分子构象。在这些实验中，当溶解温度升高到90°C时，NaHA分子表现出从以前相当紧凑的几何形状延伸的结构。该研究还显示，当应用初步过滤过程时，NaHA的MWD存在明显差异。
Molecular weight distribution (MWD) and structural deformation of ultrahigh molecular weight (MW) sodium hylaluronate (105–108g/mol) were studied under different sample dissolution temperature conditions, using on-line flow field-flow fractionation (FlFFF) and multiangle light scattering (MALS). Sodium hyaluronate (NaHA) materials from sarcoma fluid have been studied by dissolving them in water at three different temperature conditions (5°C, 50°C, and 90°C). Frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), with field programming, was utilized for the separation of NaHA by MW, and on-line observation of light scattering of fractionated NaHA by MALS was performed in order to determine the MWD and molecular conformation. In these experiments, NaHA molecules exhibited an extended structure from a formerly rather compact geometry when the dissolving temperature was raised to 90°C. This study also showed a clear difference in the MWD of NaHA when a preliminary filtration process was applied.