Synthesis ofS-(Carboxymethyl)-d-cysteine by 3-chloro-d-alanine chloride-lyase ofPseudomonas putida CR 1-1
Synthesis ofS-(Carboxymethyl)-d-cysteine by 3-chloro-d-alanine chloride-lyase ofPseudomonas putida CR 1-1
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恶臭假单胞菌CR 1-1 3-氯-d-丙氨酸氯化裂解酶合成S-(羧甲基)-d-半胱氨酸
DOI：
10.1007/bf02780381
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发表时间：
1983
影响因子：
3
通讯作者：
Hideaki Yamada
中科院分区：
文献类型：
--
作者：
T. Nagasawa;H. Hosono;Haruyuki Ohkishi;Hideaki Yamada
以恶臭假单胞菌（PseudomonasputidaCR 1 1）产3-氯-d-丙氨酸氯化裂解酶（3-chloro-d-alanine chloride-lyase，3-chloro-d-alanine）和巯基乙酸乙酯（ethyl-carbonyl-methyl）-d-cysteine为原料，经β-取代反应和碱水解，合成了半合成头孢菌素MT 141的重要组分S-（羧甲基）-d-半胱氨酸。合成的S-（羧甲基）-d-半胱氨酸是从大规模反应混合物中分离出来的，并进行了物理化学鉴定。利用P. putidaCR 1-1。
S-(Carboxymethyl)-d-cysteine, which is an important component of semisynthetic cephalosporin, MT-141, was enzymatically synthesized.S-(Ethoxy-carbonyl-methyl)-d-cystein was synthesized from 3-chloro-d-alanine and ethyl thioglycolate by the β-replacement reaction of 3-chloro-d-alanine chloride-lyase fromPseudomonas putidaCR 1-1 and subsequently hydrolyzed by alkali. The synthesizedS-(carboxymethyl)-d-cysteine was isolated from a large scale reaction mixture and identified physicochemically. The reaction conditions for the synthesis ofS-(ethoxycarbonylmethyl)-d-cysteine were optimized using resting cells ofP. putidaCR 1-1.