Immobilisation of Solanum chrysotrichum plant cells within Ca-alginate gel beads to produce an antimycotic spirostanol saponin
Immobilisation of Solanum chrysotrichum plant cells within Ca-alginate gel beads to produce an antimycotic spirostanol saponin
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DOI：
10.1016/s0981-9428(00)01196-7
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发表时间：
2000-11-01
影响因子：
6.5
通讯作者：
Nava-Saucedo, JE
中科院分区：
文献类型：
--
作者：
Charlet, S;Gillet, F;Nava-Saucedo, JE
墨西哥种 Solanum chrysotrichum 的植物细胞培养物可以从易碎的愈伤组织中建立。这些细胞产生一种抗真菌螺甾烷皂苷，命名为 SC1。将植物细胞固定在海藻酸钙凝胶珠内可以增强次级代谢产物的产生。通过改变钙（0.1至0.8 mol.L(-1)）和藻酸盐（1至1.5% w/v）浓度获得的不同基质用于研究固定参数对S. chrysotrichum细胞行为的影响。在整个生长周期和各种固定条件下，测量鲜重和干重、SC1 产量和活力。重要的代谢、形态和生理调节似乎是由凝胶珠网络和微环境限制所固有的细胞间相互作用产生的。通过淀粉体观察到淀粉粒的积累。固定化的S. chrysotrichum 细胞积累了5 mg.g(-1) 干重的SC1。尽管这比游离细胞少四倍，但所需的代谢物被排泄到周围培养基中（约占总产量的 40%）。 (C) 2000 年科学与医学版 Elsevier SAS。
Plant cell cultures of the Mexican species Solanum chrysotrichum can be established from friable calli. These cells produce an antifungal spirostanol saponin designated SC1. Plant cell immobilisation within Ca-alginate gel beads can lead to an enhancement in secondary metabolite production. Different matrices, obtained by varying calcium (0.1 to 0.8 mol.L(-1)) and alginate (1 to 1.5 % w/v) concentrations were used to study the influence of immobilisation parameters on the behaviour of S. chrysotrichum cells. Throughout the growth cycle and in the various immobilisation conditions, fresh and dry weight, SC1 yield and viability were measure. Important metabolic, morphological and physiological modulation seemed to be generated by the gel bead networks and by the cell-cell interactions inherent to the microenvironmental constraints. An accumulation of starch grains was observed through amyloplasts. Immobilised S. chrysotrichum cells accumulated 5 mg.g(-1) dry weight of SC1. Although this was four times less than the free cells, the desired metabolite was excreted into the surrounding medium (around 40% of the total production). (C) 2000 Editions scientifiques et medicales Elsevier SAS.