Comparison of activity of deferoxamine with that of oral iron chelators against human neuroblastoma cell lines.
Comparison of activity of deferoxamine with that of oral iron chelators against human neuroblastoma cell lines.
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发表时间：
1989-06
期刊：
影响因子：
11.2
通讯作者：
J. Blatt;S. Taylor;G. Kontoghiorghes
中科院分区：
文献类型：
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作者：
J. Blatt;S. Taylor;G. Kontoghiorghes
铁络合剂去铁胺已显示出对神经母细胞瘤细胞的细胞毒性。在这项研究中，我们检测了几种潜在较便宜的口服螯合剂的体外抗神经母细胞瘤活性。在摩尔比的基础上，1-羟基吡啶-2-硫氨酸(欧马丁)的细胞毒性是去铁胺的100倍。1，2-二甲基-3-羟基吡啶-4-酮也可引起明显的细胞死亡，但其摩尔浓度比去铁胺所需的浓度高得多。2，3-二羟基苯甲酸在一定浓度范围内对神经母细胞瘤细胞活力无影响。与去铁胺和1，2-二甲基-3-羟基吡啶-4-酮的作用不同，奥马定引起的作用在孵育24小时内是永久性的，离子铁的存在不会显著改变这些作用，并且与细胞周期中处于S-G2-M期的细胞比例增加有关。在这些体外研究的基础上，我们认为，特别是奥马定和一般的铁络合剂，单独使用或作为细胞周期募集剂与标准的细胞周期特异性药物一起使用，是一种值得进一步研究的癌症治疗方法。
The iron chelator, deferoxamine, has demonstrated cytotoxicity against neuroblastoma cells. In this study we examined the in vitro antineuroblastoma activity of several potentially less expensive oral chelating agents. On a mole for mole basis, 1-hydroxypyridine-2-thionine (omadine) had 100 times the cytotoxicity of deferoxamine. 1,2-Dimethyl-3-hydroxypyrid-4-one also caused demonstrable cell death but at considerably higher molar concentrations than those required for deferoxamine. 2,3-Dihydroxybenzoic acid had no effect on neuroblastoma cell viability over a range of concentrations. In contrast to the effect of both deferoxamine and 1,2-Dimethyl-3-hydroxypyrid-4-one, those due to omadine were permanent within 24 hours of incubation, were not significantly altered by the presence of ionic iron, and correlated with an increase in the percentage of cells in the S-G2-M phases of the cell cycle. On the basis of these in vitro studies, we believe that the use of omadine in particular and iron chelators in general, by themselves or as cell cycle-recruiting agents together with standard cell cycle specific drugs, is an approach to the treatment of cancer worth further investigation.