Impact of titanium ions on osteoblast-, osteoclast- and gingival epithelial-like cells
Impact of titanium ions on osteoblast-, osteoclast- and gingival epithelial-like cells
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DOI：
10.1016/j.jpor.2009.07.003
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Mimura, Sumiyo
中科院分区：
文献类型：
--
作者：
Mine, Yuichi;Makihira, Seicho;Mimura, Sumiyo
目的：研究钛离子对牙种植体周围成骨细胞、破骨细胞和牙龈上皮样细胞活性、分化及骨吸收相关基因表达的影响。进行MTS测定以评价成骨细胞样MC 3 T3-E1、破骨细胞样RAW264.7和上皮细胞样GE-1细胞的活力。结果：1-9 ppm浓度的Ti离子对MC 3 T3-E1、RAW264.7和GE-1细胞的存活率影响不大，而20 ppm浓度的Ti离子对所有细胞的存活率均有显著影响。RT-PCR和实时定量RT-PCR数据分析显示，9 ppm的Ti离子显著抑制MC 3 T3-E1中Runx 2、Osterix和I型胶原的表达。在RAW264.7中，Ti离子对RANKL增强的TRAP和组织蛋白酶K的mRNA水平无影响。1-9 ppm范围内的Ti离子对GE-1中RANKL和OPG的mRNA水平没有影响，而9 ppm范围内的Ti离子增强了MC 3 T3-E1中这些基因的表达。这些结果表明，钛离子对成骨细胞和破骨细胞的活力以及成骨细胞或骨细胞的分化都显示出生物学效应，这可能影响牙种植体的预后。(C)2009年日本口腔修复学会。由Elsevier爱尔兰出版。All rights reserved.
Purpose: To investigate the effects of titanium (Ti) ions on the cell viability, the cell differentiation and the gene expressions related to bone resorption including Receptor Activator of NF-kB Ligand (RANKL) and Osteoprotegerin (OPG) in the tissues around dental implants, the osteoblast-,osteoclast-, and gingival epithelial-like cells were exposed to Ti ions.Methods: An MTS assay was carried out to evaluate the viabilities of osteoblast- like MC3T3-E1, osteoclast-like RAW264.7 and epithelial celllike GE-1 cells. The gene expressions in these cells were analyzed by the use of RT-PCR and real-time quantitative RT-PCR.Results: Ti ions in the concentration range 1-9 ppm had little effect on the viabilities of MC3T3-E1, RAW264.7 and GE-1, whereas 20 ppm Ti ions significantly decreased the viabilities of all cells. Analyses of RT-PCR and real-time quantitative RT-PCR data revealed that Ti ions at 9 ppm remarkably inhibited the expressions of Runx2,Osterix and type I collagen in MC3T3-E1. In RAW264.7, Ti ions showed no effects on the levels of mRNAs for TRAP and cathepsin K enhanced by RANKL. Ti ions at the range of 1-9 ppm showed no effects on the levels of mRNAs for RANKL and OPG in GE-1, while Ti ions at 9 ppm enhanced the expression of these genes in MC3T3-E1.Conclusions: These results, taken together, suggested that Ti ions show the biological effects, both on the viabilities of osteoblast and osteoclast and on the differentiation of either the osteoblastic or osteoclastic cells, which may influence the prognosis of dental implants. (C) 2009 Japan Prosthodontic Society. Published by Elsevier Ireland. All rights reserved.