Transgenerational DNA Methylation Changes in Daphnia magna Exposed to Chronic γ Irradiation.
Transgenerational DNA Methylation Changes in Daphnia magna Exposed to Chronic γ Irradiation.
复制标题
慢性 γ 辐射下大型溞的跨代 DNA 甲基化变化。
DOI：
10.1021/acs.est.7b05695
复制
发表时间：
2018
影响因子：
11.4
通讯作者：
F. Alonzo
中科院分区：
文献类型：
--
作者：
M. Trijau;J. Asselman;O. Armant;C. Adam;K. D. De Schamphelaere;F. Alonzo
我们的目的是研究大水蚤（Daphnia magna）在25天的慢性外γ照射（F0代暴露于6.5 μGy·h-1或41.3 μGy·h-1）后的表观遗传变化，以及它们在随后的恢复世代，即F2（在F1胚胎中作为种系细胞暴露）和F3（第一代真正未暴露）中的潜在遗传。观察对存活、生长和繁殖的影响，并提取DNA进行全基因组亚硫酸盐测序。结果表明，对F0的繁殖有影响，但对后代F1、F2和F3无影响。相比之下，我们观察到每代特定CpG位置的显著甲基化变化与剂量率无关，其中大多数是低甲基化。其中一些变化在剂量率和代际之间是共同的。相关的基因功能包括先前被证明在暴露于电离辐射期间起作用的基因家族和基因。在F2代和F3代之间检测到的常见甲基化变化清楚地表明，表观遗传修饰可以传递给未暴露的代，最有可能通过种系，具有潜在的环境风险。
Our aim was to investigate epigenetic changes in Daphnia magna after a 25-day chronic external γ irradiation (generation F0 exposed to 6.5 μGy·h-1 or 41.3 mGy·h-1) and their potential inheritance by subsequent recovering generations, namely, F2 (exposed as germline cells in F1 embryos) and F3 (the first truly unexposed generation). Effects on survival, growth, and reproduction were observed and DNA was extracted for whole-genome bisulfite sequencing in all generations. Results showed effects on reproduction in F0 but no effect in the subsequent generations F1, F2, and F3. In contrast, we observed significant methylation changes at specific CpG positions in every generation independent of dose rate, with a majority of hypomethylation. Some of these changes were shared between dose rates and between generations. Associated gene functions included gene families and genes that were previously shown to play roles during exposure to ionizing radiation. Common methylation changes detected between generations F2 and F3 clearly showed that epigenetic modifications can be transmitted to unexposed generations, most likely through the germline, with potential implications for environmental risk.