Changes in the H-1 histone complement during myogenesis. II. Regulation by differential coupling of H-1 variant mRNA accumulation to DNA replication.
Changes in the H-1 histone complement during myogenesis. II. Regulation by differential coupling of H-1 variant mRNA accumulation to DNA replication.
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DOI：
10.1083/jcb.101.1.175
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发表时间：
1985-07
期刊：
影响因子：
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通讯作者：
Gordon JS
中科院分区：
文献类型：
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作者：
Winter E;Palatnik CM;Williams DL;Coles LS;Wells JR;Gordon JS
我们已经证明，四个鸡H-1‘S在体外成肌过程中比例的变化主要是它们的合成与DNA复制的差异耦合的结果(见上一篇论文)。我们在这里表明，四个主要的鸡H-1‘S是由不同的mRNAs编码的，这些mRNAs指定了初级氨基酸序列变体。H-1变异mRNAs的积累在不同程度上与DNA复制有关。编码H-1c(在未分裂的肌肉细胞中相对于另一个H-1‘S的H-1变异体)的基因水平是完全解偶联的。相反，编码H-1‘S a、b和d(在未分裂的肌肉细胞中水平降低)的mRNAs水平更紧密地耦合在一起。多聚腺苷酸化不参与解偶联DNA复制中H-1c基因的积累。
We have shown that changes in proportions of the four chicken H-1's during in vitro myogenesis are primarily the result of differential coupling of their synthesis to DNA replication (see the previous paper). We show here that the four major chicken H-1's are encoded by distinct mRNAs which specify primary amino acid sequence variants. Accumulation of the H-1-variant mRNAs is coupled to DNA replication to different extents. The level of mRNA encoding H-1c (the H-1 variant that increases relative to the other H-1's in nondividing muscle cells) is completely uncoupled. In contrast, the level of mRNAs encoding H-1's a, b, and d (which have levels that decrease in nondividing muscle cells) are more tightly coupled. Polyadenylation is not involved in uncoupling H-1c mRNA accumulation from DNA replication.