Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus:: characterisation and role in organophosphate resistance
Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus:: characterisation and role in organophosphate resistance
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DOI：
10.1016/s0965-1748(98)00034-4
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发表时间：
1998-08-01
影响因子：
3.8
通讯作者：
Barker, SC
中科院分区：
文献类型：
--
作者：
Baxter, GD;Barker, SC
乙酰胆碱酯酶是有机磷和氨基甲酸酯类农药的靶标。在澳大利亚，对有机磷的抵抗力在牛的微小牛蜱中很普遍。我们已经从B.microplus中分离到了一个乙酰胆碱酯酶基因，它编码一个62 kDa的蛋白质。预测的氨基酸序列包含乙酰胆碱酯酶所特有的所有残基。在5‘端和3’端都检测到转录本的选择性剪接。在5‘端的选择性剪接将导致两个蛋白质相差六个氨基酸。这是首次报道胆碱酯酶N端编码区的选择性剪接。在耐有机磷菌株中未检测到乙酰胆碱酯酶基因的点突变。文中还讨论了微型杆菌对有机磷的抗药性的其他解释。(C)1998爱思唯尔科学有限公司。保留所有权利。
Acetylcholinesterase is the target of organophosphate and carbamate pesticides. Organophosphate resistance is widespread in the cattle tick, Boophilus microplus, in Australia. We have isolated a cDNA of acetylcholinesterase from B. microplus and show that it would encode a protein 62 kDa in size. The predicted amino acid sequence contains all the residues characteristic of an acetylcholinesterase. Alternative splicing of the transcript was detected at both the 5' and 3' ends. Alternative splicing at the 5' end would result in two proteins differing by six amino acids. This is the first report of alternative splicing of the N-terminal coding region in a cholinesterase. No point mutations were detected in the acetylcholinesterase gene from organophosphate resistant strains of B. microplus. Alternative explanations for resistance to organophosphates in B. microplus are discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.